These competent cells are optimized for protein expression from T7 promoters. All are induced with IPTG or L-arabinose and come in the convenient One Shot® format, allowing for transformation and recovery in a single tube.

Which Competent Cell Strain is Right for You?

  High level expression of recombinant proteins Highest protein expression from low copy plasmids Highest protein expression from high copy plasmids Optimized or ideal for toxic proteins
Increased mRNA stability (mutated rne gene) yesyes
Expression of known toxic genes  yes
Top Selleryes 
Best expression vector formatpET-based expression vector (low copy number T7-based plasmids)pET-based expression vector (low copy number T7-based plasmids)pCR® T7-based expression vector (high copy number, T7-based plasmids)ANY
T7-based expression vector
Inducing agentIPTG IPTG IPTG Arabinose or Arabinose & IPTG
One Shot® formatOne Shot® One Shot® One Shot® One Shot®
Kit contains20 tubes x 50 µL20 tubes x 50 µL20 tubes x 50 µL20 tubes x 50 µL
Price per transformation$13.40 – $17.90$17.50$17.75$17.15
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