Electrocompetent cells work using the electroporation process. Electrical pulses created pores that allows genetic material to permeate the bacterial membrane. Invitrogen offers a variety of electrocompetent E. coli cells to reliably clone your DNA with high efficiency. The convenient packaging formats and a variety of electroporation cuvettes will fulfill all of your electrocompetent cell transformation requirements.

Electroporation Cuvettes

Electroporation Cuvettes Choose the right electroporation cuvette for your strain. Available in a variety of sizes.

Electrocompetent cells available

 

Cell Strain

Description Application Transformation Efficiency  
MegaX DH10B™ T1R Electrocomp™ Cells

MegaX DH10B™ T1R Electrocomp™ cells are the highest-efficiency electrocompetent cells.

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Ideal for highly demanding cloning and library construction applications>3 x 1010 cfu/μgOrder Now
ElectroMAX™ DH10B™ T1 R Cells

High efficiency cells derived from DH10B strain. tonA genotype for T1 and T5 phage resistance.Efficient cloning of prokaryotic and eukaryotic genomic DNA. Efficient plasmid rescue from eukaryotic genomes.>1 × 1010 cfu/μgOrder Now
ElectroMAX™DH10B™ Cells


Derived from the DH10B™ strain in a high efficiency electrocompetent format. Also available in bulk format.Efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes.>1 × 1010 cfu/μgOrder Now
ElectroMAX™
DH5α-E™ Cells
Derived from the DH5α™ strain. Suitable for transformation by electroporation.Procedures requiring high transformation efficiencies, such as cDNA library generation or with limited input DNA.>1 × 1010 cfu/μgOrder Now
One Shot® TOP10 Electropcomp™ CellsGenetically similar to the DH10B strain. Available in single-use One Shot® format.Used for general cloning applications. Found in many TOPO® cloning and expression kits.>1 × 1010 cfu/μgOrder Now
TOP10 Electropcomp™ CellsGenetically similar to the reliable DH10B strain.Used for general cloning applications. Found in many TOPO® cloning and expression kits.>1 × 1010 cfu/μgOrder Now
TOP10F’ Electropcomp™ CellsGenetically similar to the reliable DH10B strain with the F’ genotype. Used for general cloning applications. >1 × 1010 cfu/μgOrder Now
ElectroMAX™ DH12S™ CellsA derivative of DH10B™ are suitable for transformation by electroporation.A unique genotype to support production of exceptionally clean ssDNA.>1 × 1010 cfu/μgOrder Now
ElectroMAX™ Stbl4™ Competent Cells Suitable for transformation by electroporation. Specifically designed for cloning unstable inserts.>5 × 109 cfu/μgOrder Now

Highest-efficiency electrocompetent cells

MegaX DH10B™ T1R Electrocomp™ Cells are the highest-efficiency electrocompetent cells available (Figure 1), with a guaranteed three-fold greater number of colonies per transformation (>3 x 1010 cfu/μg of pUC control DNA). They are ideal for highly demanding cloning and library construction applications. MegaX cells have the same genotype as the widely used DH10B™ T1R strain, including tonA to prevent T1 and T5 lytic phage infection and safeguard your valuable clones and libraries; however, they are manufactured using an improved process that results in a significantly higher transformation efficiency. Available in the bulk Electrocomp™ format.

 

 

Figure 1: MegaX  DH10B™  T1R  Electrocomp™  cells consistently outperform the competition. L: E.cloni®  10G SUPREME cells (Lucigen); G:GC10™  Thunderbolt™ cells  (GeneChoice);  S: ElectroTen-Blue®  electroporation-competent cells (Stratagene).

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