Clonase™ Enzymes – mediating the recombination reaction

Mediating the Recombination Reaction

As explained, the core of Gateway® cloning is the Entry vector. Once the Entry clone is ready, the gene of interested is easily shuttled to a secondary plasmid, the Destination vector. This reaction is mediated by a robust enzyme mixture called LR Clonase®, which contains the necessary protein activity to excise the gene of interest from the Entry clone and integrate it into the Destination vector, which then becomes your expression clone (see figure below). Reversing this reaction simply entails performing a BP reaction with BP Clonase® enzyme mix. Both LR and BP Clonase® enzyme mixtures are easy-to-use master mix formats ensuring consistency and reliability from reaction to reaction.

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Types of Clonase® Enzymes

Gateway® BP Clonase™ Enzyme Mixtures

Gateway BP Clonase™ enzyme contains both Int (Integrase) and IHF (Integration Host Factor) proteins that catalyze the in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and a Donor vector (containing attP sites) to generate Entry clones. View the selection guide below to find the best BP Clonase™ enzyme for your research goals.


Gateway® LR Clonase™ Enzyme Mixes

Gateway® LR Clonase™ II enzyme mix contains a proprietary blend of Int (Integrase),  IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. We offer different formats of LR Clonase enzyme mixtures, depending on your application and desired format. View the selection guide below to find the best LR Clonase™ enzyme for your research goals.

                         Figure 1

Clonase™ Enzyme Selection Guide

Rating Enzyme Format Advantages
BestLR Clonase™ II Plus Enzyme MixSingle mix of enzyme and reaction buffer
  • Highest cloning efficiency for single and multiple fragment cloning
  • Optimized for difficult cloning reactions
  • Works with MultiSite Gateway® Pro Technology
  • Easy to use single mix format ensures enzyme stability
  • Convenient 10 µl reaction set up
Very goodLR Clonase™ II Enzyme MixSingle mix of enzyme and reaction buffer
  • High efficiency for single fragment cloning
  • Convenient master single mix format eliminates pipetting steps and error
  • Easy to use single mix format ensures enzyme stability
  • Convenient 10 µl reaction set up
GoodLR Clonase™ EnzymeEnzyme
5X reaction buffer
  • Basic cloning of only single fragments
BestBP Clonase™ II Enzyme MixSingle mix of enzyme and reaction buffer
  • High efficiency cloning for Entry Clone construction
  • Single mix format eliminates pipetting steps and error
  • Easy to use single mix format ensures enzyme stability
  • Convenient 10µl reaction set up
GoodBP Clonase™ EnzymeEnzyme
5X reaction buffer
  • Basic cloning of only single fragments

Ordering Information

Sku Name Size Price Qty
11789013 Gateway® BP Clonase® Enzyme Mix 20 reactions USD 518.00
11789021 Gateway® BP Clonase® Enzyme Mix 100 reactions USD 2,240.00
11789020 Gateway® BP Clonase® II Enzyme mix 20 reactions USD 193.00
11789100 Gateway® BP Clonase® II Enzyme mix 100 reactions USD 846.00
11791019 Gateway® LR Clonase® Enzyme mix 20 reactions USD 518.00
11791043 Gateway® LR Clonase® Enzyme Mix 100 reactions USD 2,240.00
11791020 Gateway® LR Clonase® II Enzyme mix 20 reactions USD 214.00
11791100 Gateway® LR Clonase® II Enzyme mix 100 reactions USD 882.00
12538120 LR Clonase® II Plus enzyme 20 reactions USD 502.00
12538200 LR Clonase® II Plus enzyme 100 reactions USD 1,756.00
11791023 LR Clonase II for BaculoDirect™ Kits 10 reactions USD 249.00