Gateway® Destination Vectors

Expanded Options in Versatile Gateway® Recombination Cloning

The Gateway® Cloning Technology provides a rapid and highly efficient route to protein expression, functional analysis and cloning/subcloning of DNA segments. Three new Gateway®-compatible destination vectors are now available to expand your expression options. These vectors contain the Gateway® recombination sites, allowing rapid, efficient transfer of sequences into these new expression systems using site-specific recombination. The release of these vectors increases the variety of applications in which the Gateway® Recombination Cloning Technology can be used.

Select Your Destination Vector

High Level Inducible Mammalian Expression

pT-REx™-DEST30 and pT-REx™-DEST31 are part of the T-REx™ system, the first inducible mammalian expression system to use the full-length CMV promoter providing high level, regulated expression. There is no toxicity with T-REx™ because viral transactivators are not used. Easy selection of stable cells using GENETICIN® is possible due to the presence of the Neomycin resistance gene. Additionally, pT-REx™-DEST31 contains a polyhistidine (6xHis) tag allowing for rapid purification on ProBond™ resin or detection with an Anti-His Antibody.

Regulated High-level E.coli Expression

pET™-DEST42 utilizes the T7/lac promoter for regulated, high-level expression in E. coli. The vector has the pBR322 low-copy origin of replication for minimized basal expression level. A C-terminal V5 epitope and polyhistidine (6xHis) tag allow for detection using an Anti-V5 Antibody and an Anti-His Antibody respectively.

NEW Gateway®-Compatible Destination Vectors:

Vector Expressed in Features Size
pT-REx™-DEST30MammalianCMV promoter, no viral transactivators, Neomycin resistance7544 bp
pT-REx™-DEST31MammalianCMV promoter, no viral transactivators, Neomycin resistance, N-terminal
6X histidine affinity tag
7559 bp
pET™-DEST42E. coliT7 promoter, C-terminal V5 epitope, C-terminal
6X histidine affinity tag
7440 bp

Ordering Information

Sku Name Size Price Qty
12301016 Gateway® pT-Rex™-DEST30 Vector 6 µg USD 534.00
12302014 Gateway® pT-Rex™-DEST31 Vector 6 µg USD 534.00
12276010 Gateway® pET-DEST42 Vector 6 µg USD 307.00
11828029 Gateway® Vector Conversion System with One Shot® ccdB Survival Cells 1 kit USD 510.00
12535037 PCR Cloning System with Gateway® Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells 20 reactions USD 476.00
12535029 PCR Cloning System with Gateway® Technology with pDONR™221 & OmniMAX™2 Competent Cells 20 reactions USD 394.00
12535035 Gateway® pDONR™/Zeo Vector 6 µg USD 339.00
12536017 Gateway® pDONR™221 Vector 6 µg USD 204.00
K59120 pENTR™ 5'-TOPO® TA Cloning® Kit, with One Shot® TOP10 Chemically Competent E. coli 20 reactions USD 422.00
12537102 MultiSite Gateway® Pro 2.0, for cloning two DNA fragments into a Gateway® destination vector 20 reactions USD 1,834.00
12537103 MultiSite Gateway® Pro 3.0, for cloning three DNA fragments into a Gateway® destination vector 20 reactions USD 2,165.00
12537104 MultiSite Gateway® Pro 4.0, for cloning four DNA fragments into a Gateway® destination vector 20 reactions USD 2,455.00
12537100 MultiSite Gateway® Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway® destination vector 20 reactions USD 2,655.00
12537023 MultiSite Gateway® Three-Fragment Vector Construction Kit 20 reactions USD 2,165.00