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Site-Directed Mutagenesis

The GeneArt® site-directed mutagenesis service introduces single or multiple mutations (substitutions, insertions, deletions, or truncations) into existing DNA sequences quickly and economically.

We use a targeted PCR-based process to introduce genetic variation precisely where you need it, then clone the construct into the vector of your choice. All constructs are then 100% sequence-verified and documented to help assure that the DNA you receive is exactly what you’ve requested.

 

GeneArt® Services provides a full range of cloning, optimization, and protein expression options to serve many areas of biological research and production.

Two Ways to Get Your Project Started

When setting up your project online, add “single variant” or “variant bundle” to your project and follow the instructions to enter your sequence(s)
Download the Multiple Sequence Submission Form, enter your DNA sequence request(s) using Microsoft Excel, and email it to our customer support team.

For further information regarding this service or the order process, please contact christian.kranz@lifetech.com.

What You Receive

GeneArt® Mutagenesis Service
—5 µg of plasmid DNA
—100% sequence verified and cloned into your vector 

GeneArt® Mutagenesis 6+ Service
—Discounted subcloning into your vector for 6 or more related constructs
—5 µg DNA of each construct
—100% sequence verified

Applications

  • Investigate active sites, structure-function relationships, nucleic acid–protein interactions, etc.
  • Test functional elements (promoter regions, terminator sequences, etc.)
  • Construct fusion proteins and tagged proteins
  • Design knockout constructs
  • Generate alternative splice forms
  • Conduct alanine scans to identify protein functional domains

Advantages

  • Economical alternative to synthetic gene construction for constructs smaller than ~1 kb
  • Mutations exactly where you need them, without the risk of introducing unwanted mutations
  • Unlike “quick change” strategies, the vector is not subjected to PCR—just the insert
  • Insert size can be as large as 6–8 kb
  • No mutations in the vector backbone
  • Mutations can be clustered or far apart, with the same cost and production time
  • Fast turnaround times
  • Template constructs are stored for at least 2 years at no charge and longer-term storage is available
  • Full sequence verification of insert DNA
  • Competitive pricing

References