Transcription activator–like effector nuclease (TAL, TALE, or TALEN) proteins are naturally occurring transcriptional activators secreted by Xanthomonas spp. into their plant hosts. The deciphering of the TAL effector “code” led to the engineering of designer TAL effector proteins, a new approach to genome editing. Whether through targeted nucleases for genome engineering, or by precisely directed moderators of gene expression, GeneArt® TALs are already helping advance a broad range of life science applications, including cell, molecular, and synthetic biology; drug discovery; and biofuels research.
Learn more about TALEN-based editing
Precise and extraordinarily flexible genome editing
GeneArt® TALs provide a custom DNA-binding proteins for accurate DNA targeting and precise gene editing and gene regulation. Unlike other technologies that limit the choice of targets, or provide ambiguous results, GeneArt® TAL enables the targeting of any locus in the genome. Thermo Fisher Scientific now offers TAL nucleases with new design that removes the 5’ base constraint and therefore can be designed to target any desired sequence within the genome, a technology we've termed GeneArt® PerfectMatch TALs. GeneArt® PerfectMatch TALs are offered as Gateway® entry vectors with FokI nuclease or with CMV-driven expression for ready-to-transfect expression in mammalian systems. GeneArt® Precision TALs offers site-specific delivery of nucleases, activators, repressors, chromatin modifiers, genomic labels, and cross-linking molecules. This genome editing technology is known to function in a variety of host systems, including bacteria, yeast, plants, insects, zebrafish, and mammals.
An essential tool for monitoring the efficiency of your genome editing experiments
When using genome editing tools such as CRISPRs, TALs, or zinc finger nucleases to obtain targeted mutations, it is necessary to determine the efficiency with which these nucleases cleave the target sequence prior to continuing with labor-intensive and expensive experiments. The GeneArt® Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method to determine the nuclease cleavage efficiency at a given locus.