GeneArt® TALENTranscription activator–like effector nuclease (TAL, TALE, or TALEN) proteins are naturally occurring transcriptional activators secreted by Xanthomonas spp. into their plant hosts. The deciphering of the TAL effector “code” led to the engineering of designer TAL effector proteins, a new approach to genome editing. Whether through targeted nucleases for genome engineering, or by precisely directed moderators of gene expression, GeneArt® Precision TALs are already helping advance a broad range of life science applications, including cell, molecular, and synthetic biology; drug discovery; and biofuels research.

Learn more about TALEN-based editing


Design with precision

GeneArt® Precision TALs provide a custom DNA-binding proteins for accurate DNA targeting and precise genome editing. Unlike other technologies that limit the choice of targets, or provide ambiguous results, GeneArt® Precision TAL enables the targeting of any locus in the genome. GeneArt® Precision TALs offers site-specific delivery of nucleases, activators, repressors, chromatin modifiers, genomic labels, and cross-linking molecules. This genome editing technology is known to function in a variety of host systems, including bacteria, yeast, plants, insects, zebrafish, and mammals.

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Precise genome editing for every gene locus is now within your reach

Free TALEN Design Consultation

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Custom TALEN Cell Line Service

Request information about GeneArt® Precision TALs

 

Ordering GeneArt® Precision TALs

The fastest and easiest way to design, edit, optimize, and order GeneArt® Precision TALs is through the GeneArt® portal.

Order online

The ordering process is simple; just follow the three steps below:

  1. Select the functionality (effector domain) of your TAL from Table 1. For details, refer to the ‘TALEN functionality overview
  2. Select the product name from Table 1.
  3. Then place your order through our online design tool within the GeneArt® portal, or you can download and complete the TALEN order form and email it to geneartsupport@lifetech.com.

If you have a question or need free design consultation, contact us here and we’ll be happy to assist.

We will ship you a clone with a verified, optimized sequence approximately 2 weeks after confirming your order.

Table 1: Available effector domains

Product DNA-binding domain Effector domain Functionalities Applications
Native TALEN™ Fok1 Native Fok1 endonuclease Gene targeting
  • Silencing
  • Incorporation of exogenous DNA
Truncated TALEN™ Fok1 Truncated Fok1 endonuclease Gene targeting
  • Silencing
  • Incorporation of exogenous DNA
Native TAL VP16 activator Native VP16 activator Activation of transcription
  • Increasing the expression level of endogenous gene isoforms
Native TAL VP64 activator Native VP64 activator Activation of transcription
  • Increasing the expression level of endogenous gene isoforms
TAL repressor Modified KRAB repressor Epigenetic repression of transcription
  • Heritable knockdown of gene expression
Native TAL MCS Native (MCS) Steric repression & custom design
  • Transient knockdown of gene expression
  • Target any locus in the genome with the effector domain of your choice
Truncated TAL MCS Truncated (MCS) Steric repression & custom design
  • Transient knockdown of gene expression
  • Target any locus in the genome with the effector domain of your choice

KRAB: Krüppel associated box; MCS: multiple cloning site; TAL: transcription activator–like (DNA-binding domain).

GeneArt® Genomic Cleavage Detection Kit

An essential tool for monitoring the efficiency of your genome editing experiments

When using genome editing tools such as CRISPRs, TALs, or zinc finger nucleases to obtain targeted mutations, it is necessary to determine the efficiency with which these nucleases cleave the target sequence prior to continuing with labor-intensive and expensive experiments. The GeneArt® Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method to determine the nuclease cleavage efficiency at a given locus.