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Tools for flexible protocols, and fast, high-quality results

  • Speed—clone up to 10 DNA fragments simultaneously; assemble existing fragments without restriction digest or PCR amplification
  • Precision—no extra sequences; clone what you want where you want
  • Flexibility—use our linearized vectors or any vector of your choice
  • Simplicityfree online tool  guides you through planning, design and assembly of your construct and PCR primer design, if needed

Expert Vector Construction: The GeneArt® Seamless Cloning and Assembly Kit eliminates multiple rounds of restriction enzyme analysis and digestion, DNA end-repair, de-phosphorylation, ligation, enzyme inactivation and clean-up, and loss of precious DNA. It’s what experts at Life Technologies use to make vectors, and they save 3-4 weeks versus traditional RE cloning methods.

Which Seamless Cloning & High-Order Genetic Assembly Kit is Right for You?

  Assemble up to 4 fragments Assemble up to 4 fragments Assemble up to 10 fragments Assemble up to 4 fragments
Cloning Efficiency
  • >90%- 1 Fragment, 5 kb each
  • >70% - 4 Fragments, 1 kb each

  • 90% - single 5 kb DNA
  • 70% - 4 Fragments, 1 kb each
  • 40% - 4 DNA Fragments, 2 kb each
  • >90% - 5 DNA Fragments, 10 kb each
  • >90% - 10 DNA Fragments, 5 kb each
  • 50% - 10 DNA Fragments, 10 kb each
  • >90%- 4 Fragments, 5 kb each
  • >90% - 4 Fragments, 10 kb each
All-in-one Enzyme/Buffer Mix yesyes
Good for HTP yes yes
# of Fragments Cloned Simultaneously Up to 4Up to 4Up to 10Up to 4
Maximum Assembled Product Size Up to 13 kb (with vector) Up to 13 kb (with vector) Up to 110 kb (with vector) Up to 40 kb (with vector)
Time to Clone Multiple Fragments Typically in half a day 1 day 3 days Typically in half a day
Replication Host E. coli E. coli S. cerevisae MaV203 E. coli
Assemble Fragments w/o End Sequence Homology w/o PCR yes
Price/Reaction $14.95 $19.85 $43.22 $22.25
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GeneArt® Web-Based Design Tool

Guides the assembly of fragments, checks for potential issues, designs DNA oligonucleotides and presents an annotated sequence and graphical representation of the final assembled molecule.
         GeneArt® Web-Based Design Tool

NEW Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications