Seamless Cloning & Genetic Assembly
Tools for flexible protocols, and fast, high-quality results
- Speed—clone up to 10 DNA fragments simultaneously; assemble existing fragments without restriction digest or PCR amplification
- Precision—no extra sequences; clone what you want where you want
- Flexibility—use our linearized vectors or any vector of your choice
- Simplicity—free online tool guides you through planning, design and assembly of your construct and PCR primer design, if needed
Expert Vector Construction: The GeneArt® Seamless Cloning and Assembly Kit eliminates multiple rounds of restriction enzyme analysis and digestion, DNA end-repair, de-phosphorylation, ligation, enzyme inactivation and clean-up, and loss of precious DNA. It’s what experts at Life Technologies use to make vectors, and they save 3-4 weeks versus traditional RE cloning methods.
Which Seamless Cloning & High-Order Genetic Assembly Kit is Right for You?
|Assemble up to 4 fragments||Assemble up to 4 fragments||Assemble up to 10 fragments||Assemble up to 4 fragments|
|All-in-one Enzyme/Buffer Mix|
|Good for HTP|
|# of Fragments Cloned Simultaneously||Up to 4||Up to 4||Up to 10||Up to 4|
|Maximum Assembled Product Size||Up to 13 kb (with vector)||Up to 13 kb (with vector)||Up to 110 kb (with vector)||Up to 40 kb (with vector)|
|Time to Clone Multiple Fragments||Typically in half a day||1 day||3 days||Typically in half a day|
|Replication Host||E. coli||E. coli||S. cerevisae MaV203||E. coli|
|Assemble Fragments w/o End Sequence Homology w/o PCR|
|Order Now||Order Now||Order Now||Order Now|
GeneArt® Web-Based Design Tool
Guides the assembly of fragments, checks for potential issues, designs DNA oligonucleotides and presents an annotated sequence and graphical representation of the final assembled molecule.
|GeneArt® Web-Based Design Tool|
NEW Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications