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GeneArt® Seamless Cloning and Assembly enables cloning of up to 4 DNA fragments simultaneously into virtually any linearized vector in 30 minutes, without extra DNA sequences, restriction endonucleases or ligation.

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GeneArt® Seamless Cloning & Assembly

The GeneArt® Seamless Cloning and Assembly Enzyme mix , the GeneArt® Seamless Cloning and Assembly Kit, and the GeneArt® Seamless PLUS Cloning and Assembly kit enable cloning of DNA fragments into virtually any linearized vector, without extra sequences, or restriction endonuclease digestion and ligation. The proprietary enzyme mix recognizes and precisely assembles the DNA fragments sharing at least 15-base pair (bp) end homology created by PCR amplification in a simple ~30 minute room temperature reaction. The DNA Oligo Designer guides users through experimental design. Learn more about the differences between these kits here

GeneArt® Seamless Cloning and Assembly Kits work great together with GeneArt® Strings™ DNA Fragments. This service offers custom-made DNA fragments up to 3,000 bp ready for cloning and assembly and shipped in only 5-8 business days.


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  This figure outlines the GeneArt® Seamless Cloning and Assembly protocol for assembling up to four DNA fragments and a vector simultaneously and in precise order.

 

gene art work flow
  This figure outlines the GeneArt® Seamless PLUS Cloning and Assembly protocol for assembling up to four DNA fragments and a vector simultaneously and in precise order.

Expert Vector Construction


  The GeneArt® Seamless Cloning and Assembly Kit eliminates multiple rounds of restriction enzyme analysis and digestion, DNA end-repair, de-phosphorylation, ligation, enzyme inactivation and clean-up, and loss of precious DNA. It’s what experts at Life Technologies use to make vectors, and they save 3-4 weeks versus traditional RE cloning methods.  

Leader in Multiple Fragment Assembly Efficiency

 The GeneArt® Seamless Cloning and Assembly Kit delivers high cloning efficiencies across a wide range of fragment sizes and numbers. A comparative analysis against competitor 'I' reveals the advantage of using GeneArt® Seamless Cloning and Assembly when 3 or more DNA fragments are cloned simultaneously.

Reliable and Easy to Use


GeneArt® Seamless Cloning and Assembly is an easy and reliable method for routine cloning needs. Three scientists assembled four 1 kbp DNA fragments following the GeneArt® Seamless Cloning and Assembly protocol with an average cloning efficiency of 95.8%.

Achieving Optimal Cloning Efficiency

 The GeneArt® Seamless Cloning and Assembly Kit provides high cloning efficiency across a wide range of fragment sizes and fragment numbers. Using the AccuPrime® Pfx SuperMix to amplify the inserts and/or vector can significantly increase the cloning efficiency in a multifragment assembly as compared to the Platinum® PCR SuperMix High Fidelity.

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Eliminate complicated, frustrating traditional cloning methods and begin high efficiency cloning of multiple DNA fragments today!