When TOPO® cloning technology was launched, it sparked a cloning revolution. Over the past decade, TOPO® technology has become the most widely used cloning method in the world with over 20,000 citations.

TOPO® cloning technology allows you to perform bench-top cloning reactions in just five minutes—with up to 95% recovery of your desired clone. So you can count on having the clone you need for downstream experiments.

Meet the inventor of TOPO® cloning technology >

Choose the right TOPO® cloning solution for you

TOPO® blunt-end cloning

NEW: 25% extra reactions

For the direct insertion of blunt-end PCR products amplified with proofreading thermostable polymerases into a plasmid vector.

NEW: 25% extra reactions

For the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning.

For easy, efficient cloning of long (3–10 kb) PCR products using the pCR®-XL-TOPO® vector.

TOPO® Kits without competent cells

NEW: 25% extra reactions

Gateway® Recombinant Cloning with TOPO® Technology

Expression cloning with TOPO® Technology

Meet the Inventor

Find out how the revolutionary TOPO® cloning was developed with Dr. John Chestnut, research fellow in Synthetic Biology RND.

Dr. Jon Chesnut
TOPO® Cloning Technology