Quick and efficient PCR cloning with TOPO® TA cloning

Clone PCR-amplified DNA fragments (blunt or A-overhang) directly into a choice of over 40 subcloning, sequencing, or expression vectors in just 5 minutes—and obtain up to 95% recombinant clones.

  • Quick—avoid inefficient ligation and laborious searches for appropriate restriction enzymes
  • Efficient—minimize colony screening by obtaining up to 95% recombinant clones
  • Troubleshooting tool—if it can’t be cloned with TOPO® cloning technology, it can't be cloned

Save 20% off on selected TOPO-TA Cloning Kit

 

Description

TOPO-TA Box 1, PCR2.1

TOPO TA for sequencing

TOPO ZERO Blunt PCR Cloning kit

Cat. No.

450641

450030

450245

 

Use promo codes: US P1074284 and CA P1074285 when ordering and you will automatically receive the discount at time of purchase. *Terms and conditions apply

 

TOPO® cloning protocol


Figure 1. The TOPO® cloning protocol. TOPO® PCR cloning requires just three easy steps. Simply combine your PCR product and a TOPO® cloning vector in the provided solution, wait five minutes, then transform E. coli.

Steps TOPO® TA Cloning Kits TA/UA Cloning Restriction enzyme or cut-back cloning
Use of existing primers? Yes Yes No
Vector is ready for cloning? Yes Yes No
Ligation regents included? Yes Yes No
Prepare or purchase competent cells separately? No, included Purchase: 0 hr
Prepare: up to 6 hr
Purchase: 0 hr
Prepare: up to 6 hr
Ligation time 5 min 1 hr 2 - 23 hr
Recombination efficiency Up to 95% 60 - 80% ~60%
Time required for cloning 5 min 1 - 12 hr 2 - 23 hr

*Terms and Conditions:

This promotion is open to customers in the US and Canada. Discount will apply to orders received by Life Technologies no later than July31, 2014. Discount is valid only for the following SKUs: 450641, 450030 and 450245. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.