The ChargeSwitch® gDNA Mini Bacteria Kit provides a fast and reliable magnetic bead–based method for purifying genomic DNA (gDNA) from both gram-negative and gram-positive bacteria. Featuring a simple one-tube protocol that can be completed in less than 15 minutes after preparing lysates. This kit features:
- A simple one-tube protocol that can be completed in less than 15 minutes after preparing lysates
- Efficient DNA purification from individual colonies or 0.1 to 0.5 mL of overnight cultures (Figure 1)
- Yields of up to 12 µg of genomic DNA per prep
Figure 1. Efficient DNA purification from low numbers of bacteria. Genomic DNA was purified from a variety of Salmonella typhimurium samples using the ChargeSwitch® gDNA Mini Bacteria Kit. Following purification, the invA gene was amplified by PCR (456 bp product), and the PCR products were run on a 1% agarose gel and stained with ethidium bromide. Lane L: 1 Kb DNA Ladder; lane P: cells pelleted from 0.5 mL of culture; lane W: 100 μL of overnight culture; lane C: a single colony picked directly from an agar plate.
Flexible DNA Purification for High-Throughput Applications
Unlike other bacterial DNA purification methods, the ChargeSwitch® gDNA Mini Bacteria Kit provides the flexibility to purify DNA from a wide range of both gram-positive and gram-negative bacteria (Figures 2 and 3). Furthermore, this kit’s protocols are designed to be compatible with standard liquid handling robots and high-throughput applications; they contain no centrifugation or filtration steps and use reagents that will not clog lines or cause vapor pressure build-up. For added convenience, all ChargeSwitch® Kits work well with the MagnaRack™ Magnetic Rack, a two-piece magnetic separation rack that enables processing of up to 24 microcentrifuge tubes at one time (Figure 4).
Figure 2. Purification of genomic DNA from gram-positive bacteria. Genomic DNA samples isolated from 20 species of enterococci using the ChargeSwitch® gDNA Mini Bacteria Kit show high A260/A280 ratios indicative of DNA purity. Data kindly provided by Dr. J. Caplin, School of the Environment, University of Brighton, UK.
|Figure 3. Purification of genomic DNA from gram-negative bacteria. Genomic DNA was purified from two species of gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using the ChargeSwitch® gDNA Mini Bacteria Kit in parallel with a kit from Competitor G. The purified DNA was then run on a 1% agarose gel and stained with ethidium bromide. Lane 1: 100 ng lambda DNA; lane 2: 500 ng lambda DNA; lanes 3 and 4: E. coli DNA purified with the CST® Kit; lanes 5 and 6: E. coli DNA purified with Competitor G’s kit; lanes 7 and 8: P. aeruginosa DNA, CST® Kit; lanes 9 and 10: P. aeruginosa DNA, Competitor G’s kit. The ChargeSwitch® gDNA Mini Bacteria Kit can also be used to purify genomic DNA from gram-positive bacteria (Figure 3); however, Competitor G’s kit, which employs a salting-out/centrifugation method, is unsuitable for use with gram-positive bacteria.|
Figure 4. Process 24 samples at a time using the MagnaRack™ Magnetic Rack. The ChargeSwitch® gDNA Mini Bacteria Kit works well with the MagnaRack™ Magnetic Rack, which has precisely aligned neodymium magnets that enable processing of up to 24 microcentrifuge tubes in a variety of magnetic bead–based separations.
|Other Products of Interest
In addition to DNA and RNA isolation products, Life Technologies offers an expansive portfolio of solutions for analysis and quantitation of tissue and cultured cell genomic DNA.
For Research Use Only. Not for use in diagnostic procedures.