PCR Clean-Up From Complex Mixtures of DNA

PCR clean-up is a routine but time-consuming laboratory procedure. Now you can use an easier, faster, safer method and get superior results. Purify DNA using a simple and rapid PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.

Isolated DNA is ready for sequencing, PCR, transcription, mapping, cloning, and labeling. We offer a wide range of Invitrogen™ PCR clean-up kits, plus the support you may need to get high yields of pure DNA (Figure 1).

ChargeSwitch®-Pro kits Figure 1. ChargeSwitch®-Pro kits simplify PCR clean-up. Sequencing results from amplicons purified using the ChargeSwitch®-Pro PCR Clean-Up Kit. The ChargeSwitch®-Pro PCR Clean-Up Kit and QIAquick® PCR Purification Kit were used according to the manufacturers’ directions. A purified 2 kb sample (100 ng) from each purification was used in BigDye® Terminator sequencing reactions with the T7 primer. (A) Electropherogram of a sample purified with the ChargeSwitch®-Pro kit. (B) Phred 20 scores averaged from triplicate samples.

Which PCR product clean-up kit is right for you?

Quick and Efficient removal of by-products Ideal for concentrating low yield PCR products Simple, reliable, rapid method, 96-well format Rapid, one tube, 5 minute protocol for high recovery of DNA Complete kit with no harsh chemicals for fast, hassle-free automatable setup
FormatSpin/vacuum columnSpin/vacuum column96-well plate (vacuum/centrifuge)Magnetic beadsSpin/vacuum column
Protocol time15 min15 min15 min5 min5 min
Sample volume50–100 µL50–100 µL50–100 µL25–50 µL
DNA fragment size100 bp–15 kb100 bp–15 kb100 bp–15 kb90 bp–40 kb90 bp–40 kb
Binding capacityUp to 40 µgUp to 40 µgUp to 40 µg~25 µg per mg beads~25 µg per mg beads
Downstream ApplicationSequencing, Sequencing (Next Generation), Nucleic Acid Labeling, PCR, CloningSequencing, Nucleic Acid Labeling, PCR, CloningSequencing, CloningSequencing, Sequencing (Next Generation), Microarray Analysis, PCR, CloningSequencing, Sequencing (Next Generation), Microarray Analysis, PCR, Cloning
High Throughput CompatibilityYesNoYesYesNo
Price per prep


$1.59 $1.81
Pack size50 preps
250 preps
50 preps
250 preps
4 plates100 preps
960 preps

50 preps
250 preps

Cat. No.K310001
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PureLink® Combo Kit

If you do PCR clean-up and gel extractions, consider our PureLink® combo kit that allows you to do either process with a single kit.

 PureLink® Quick Gel Extraction and PCR Purification Combo Kit 

For Research Use Only. Not for use in diagnostic procedures.