Agarose gel electrophoresis remains the most widely used technique for separating nucleic acid fragments because it is a simple technique that is nontoxic and offers a broad separation range. The size of the gel pores can be controlled by simply adjusting the agarose concentration to prepare gels appropriate for the separation of a wide range of different-size nucleic acid molecules. The migration of nucleic acids in agarose gels is also affected by the choice of running buffer and the applied voltage.

Life Technologies offers convenient reagents for agarose gel electrophoresis including hassle-free precast E-Gel® agarose gels, safe and sensitive DNA stains, DNA ladders, and UltraPure™ reagents to cast agarose gels yourself.

E-Gel® Precast Agarose Gel Systems

E-Gel® system 

The E-Gel® system is a bufferless system for agarose gel electrophoresis, viewing, and documentation. Samples are run in E-Gel® pre-cast agarose gels that include electrodes, and are packaged in a dry, disposable, UV-transparent cassette. E-Gel® gels are run in a base that includes a combined electrophoresis chamber and power supply. The precast agarose gels contain SYBR® Safe stain, ethidium bromide, proprietary fluorescent DNA stain, or no DNA gel stain.

Advantages of the E-Gel® system:

  • Offers fast, safe, consistent, high-resolution electrophoresis
  • Eliminates the need to prepare agarose gels and buffers, and to stain gels
  • Compatible with most robotic systems for high-throughput electrophoresis

UltraPure™ Agarose and Reagents

UltraPure™ agarose products 

Life Technologies offers a wide range of UltraPure™ agarose products for preparing your own gels for nucleic acid electrophoresis. All UltraPure™ reagents are made from the highest purity biochemicals for maximum reliability and superior performance.

Every UltraPure™ lot is tested to help ensure:

  • Low electroendosmosis to minimize internal convection and band spreading during electrophoresis, resulting in sharp band resolution
  • No detectable DNase or RNase activity

DNA Ladders and Markers

DNA Ladders and Markers Life Technologies offers a broad selection of DNA ladders and markers ranging from 10 bp to 50 Kb for accurate analysis of linear double-stranded DNA in agarose gels. A variety of these ladders are available in the ready-to-use TrackIt™ and E-Gel®  formats, including our popular 1 Kb Plus DNA Ladder and 100 bp DNA Ladder.

DNA Stains

SYBR® Safe DNA gel stain Many DNA stains are available from Life Technologies including SYBR® Safe DNA gel stain and ethidium bromide. SYBR® Safe DNA gel stain is a superior DNA stain that is nontoxic and nonmutagenic, yet has similar DNA detection sensitivity as ethidium bromide.

Agarose Gel Extraction Kits

Agarose Gel Extraction Kits Quick and easy gel extraction of DNA
Purify DNA fragments from agarose gels in less than 30 minutes.  Isolated DNA is free of proteins, dye, and agarose, and is ready to use for a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.