DNA Size Markers & Mass Ladders
DNA ladders comprising fragments of specific base pair lengths are available for estimation of molecular size from 10 bp to 12 kb. For easy determination of fragment size by visual reference, several DNA ladders include bands that are two to three times more intense than the other bands. All DNA ladders can be stained with ethidium bromide, SYBR® Safe, or other DNA stains, and several can be radiolabeled using T4 DNA polymerase or T4 polynucleotide kinase.
Ladders & Markers Selection Guide
|DNA Ladder||Base Pair (bp) Range|
|λ DNA/Hind III Fragments||125 bp–23.1 kb|
|X FF174 RF DNA/Hae III Fragments||72 bp–1.35 kb|
|pUC19/Sau3A I Digest dsDNA marker||8–955 bp|
|1Kb Plus DNA Ladder (replaces 1Kb DNA Ladder)||0.1–12 kb|
|1Kb DNA Extension Ladder||0.5–40 kb|
|10bp DNA Ladder||10–200 bp|
|25bp DNA Ladder||25–500 bp|
| 50bp DNA Ladder
|100bp DNA Ladder||0.1–1.5 kb|
|123bp DNA Ladder||123–3,075 bp|
|250bp DNA Ladder||0.25–3.5 kb|
|500bp DNA Ladder|
|Supercoiled DNA Ladder||2–16 kb|
|Low DNA Mass ladder||100–2,000 bp|
|High DNA Mass ladder||1–10 kb|
|High Molecular Weight DNA Markers|
DNA Mass Ladders
Double-stranded DNA size markers with band sizes ranging from 72 bp to 23.1 kb are available for use in agarose gel electrophoresis. These markers are suitable for estimation of fragment length.
Figure above. pUC19/Sau3A I Digest dsDNA marker.