E-Gel® SizeSelect™ Gels

The 15 minute size separation and recovery system for library construction

  • Size separate free adapters from ligated product 10X faster in 3 easy steps
  • Optimized for tight, consistent recovery of DNA fragments <1Kb in size
  • Purified DNA is compatible with library construction protocols of major generation 2 sequencing platforms
  • Use recovered DNA directly in cloning reactions

Size Select DNA in 3 Easy Steps

With E-Gel® SizeSelect™ gels, you can separate and recover DNA for library construction in 3 easy steps,

  1. Load
  2. Run
  3. Retrieve


E-Gel® SizeSelect™ Gels are double-comb, pre-cast agarose gels with a twist. Load your sample into the top row and electrophorese until your band or desired size range enters the bottom row (Figure 1). Then, easily remove the size-selected DNA with a pipette. That’s it. No additional steps are required. Use the E-Gel® iBase™ Power System, a compact, self-contained device with a built-in power supply, to run the gels. A proprietary new DNA stain incorporated into the E-Gel® SizeSelect™ gels is excitable by blue light, allowing live monitoring of DNA migration with the E-Gel® Safe Imager™, a compact blue light transilluminator designed to fit beneath the E-Gel® iBase™ Power System.



Figure 1. Size select DNA 10X faster in 3 easy steps. Samples are loaded in the top row of wells, bands separate during the gel run, and individual bands or portions of a smear are collected from the bottom row after they enter those wells. Reverse-run functionality on the E-Gel® iBase™ Power System lets you capture bands of interest even if you miss the bands when they pass through the collection wells. Watch live migration of DNA with the E-Gel® Safe Imager™ Real Time Transilluminator

Have DNA Ready for Library Construction 10 Times Faster

Separate and Recover in Just 15 Mins

The E-Gel® SizeSelect™ gels allow users to separate and recover DNA for short read fragment library construction in just 15 minutes. That's 10 times faster than using conventional gels, and purification kits. E-Gel® SizeSelect™ gels are offered in a 2% agarose concentration, and validated  for the recovery of fragments in the 150-200bp size range. Once the DNA is recovered, no additional purification step is required before proceeding to the next step in library construction. E-Gel® SizeSelect™ system has been used successfully in these generation 2 library construction protocols:

  • ABI's SOLiD Fragment Library Construction
  • ABI's SOLiD Mate Pair Library Construction
  • Illumina's Genome Analyzer Fragment Library Construction
  • Illumina's Genome Analyzer Paired-end Library Construction


The Fastest and Most Accurate

The E-Gel® SizeSelect™ system is the fastest, most accurate method for the separation and recovery of DNA fragments for library construction using a major generation 2 sequencing platform. The E-Gel® SizeSelect™ system,

  • Dramatically reduces the time required to construct libraries
  • Provides tight, consistent size recovery of fragments <1Kb in size

The Smartest Way to Gel-Purify DNA for Cloning Reactions

E-Gel® SizeSelect™ gels are the smartest way to gel-purify DNA fragments <1Kb in size for downstream cloning reactions. Purified DNA can be used directly in any cloning reaction without the need for additional purification kits. The proprietary stain incorporated in the E-Gel® SizeSelect™ gels is five times more sensitive than ethidium bromide, allowing maximal retrieval of DNA bands. Avoid the personal hazards of UV exposure by using the Safe Imager™ Real-Time Transilluminator for visualization migrating DNA bands. The blue light produced by the Safe Imager™ Real-Time Transilluminator, ensures integrity of the sample DNA which in turn maximizes cloning efficiency.

Choose E-Gel® SizeSelect™ gels for cloning fragments <1Kb and enjoy:

  • Optimal cloning results—minimal DNA damage and maximal yield
  • Time savings—have DNA ready for cloning in minutes
  • Enhanced personal safety—avoid exposure to harmful UV radiation

 

 Figure 3. Cloning efficiency with E-Gel® SizeSelect™ Gels and the Safe Imager® blue light transilluminator. PCR-generated inserts of 3 different molecular weights were cloned into pCR® 2.1 TOPO® vector using manufacturer’s protocol. Prior to cloning, the fragments were purified from either E-Gel® incorporated with ethidium bromide, or E-Gel® SizeSelect™ gels. Fragments were retrieved from E-Gels with ethidium bromide by excision using a razor blade followed by column purification. Reactions were performed in triplicates and data are presented as average values. Standard deviations are represented as error bars.

Ordering Information

Sku Name Size Price Qty
G6612ST E-Gel® SizeSelect™ Agarose Gels Starter Kit, 2% 1 kit USD 1,250.00
G661002 E-Gel® SizeSelect™ Agarose Gels, 2% 10⁄pack USD 158.00
10416014 50 bp DNA Ladder 50 µg USD 133.00