E-Gel® SizeSelect™ Gels

The 15 minute size separation and recovery system for library construction

  • Size separate free adapters from ligated product 10X faster in 3 easy steps
  • Optimized for tight, consistent recovery of DNA fragments <1Kb in size
  • Purified DNA is compatible with library construction protocols of major generation 2 sequencing platforms
  • Use recovered DNA directly in cloning reactions

Size Select DNA in 3 Easy Steps

With E-Gel® SizeSelect™ gels, you can separate and recover DNA for library construction in 3 easy steps,

  1. Load
  2. Run
  3. Retrieve


E-Gel® SizeSelect™ Gels are double-comb, pre-cast agarose gels with a twist. Load your sample into the top row and electrophorese until your band or desired size range enters the bottom row (Figure 1). Then, easily remove the size-selected DNA with a pipette. That’s it. No additional steps are required. Use the E-Gel® iBase™ Power System, a compact, self-contained device with a built-in power supply, to run the gels. A proprietary new DNA stain incorporated into the E-Gel® SizeSelect™ gels is excitable by blue light, allowing live monitoring of DNA migration with the E-Gel® Safe Imager™, a compact blue light transilluminator designed to fit beneath the E-Gel® iBase™ Power System.



Figure 1. Size select DNA 10X faster in 3 easy steps. Samples are loaded in the top row of wells, bands separate during the gel run, and individual bands or portions of a smear are collected from the bottom row after they enter those wells. Reverse-run functionality on the E-Gel® iBase™ Power System lets you capture bands of interest even if you miss the bands when they pass through the collection wells. Watch live migration of DNA with the E-Gel® Safe Imager™ Real Time Transilluminator

Have DNA Ready for Library Construction 10 Times Faster

Separate and Recover in Just 15 Mins

The E-Gel® SizeSelect™ gels allow users to separate and recover DNA for short read fragment library construction in just 15 minutes. That's 10 times faster than using conventional gels, and purification kits. E-Gel® SizeSelect™ gels are offered in a 2% agarose concentration, and validated  for the recovery of fragments in the 150-200bp size range. Once the DNA is recovered, no additional purification step is required before proceeding to the next step in library construction. E-Gel® SizeSelect™ system has been used successfully in these generation 2 library construction protocols:

  • ABI's SOLiD Fragment Library Construction
  • ABI's SOLiD Mate Pair Library Construction
  • Illumina's Genome Analyzer Fragment Library Construction
  • Illumina's Genome Analyzer Paired-end Library Construction


The Fastest and Most Accurate

The E-Gel® SizeSelect™ system is the fastest, most accurate method for the separation and recovery of DNA fragments for library construction using a major generation 2 sequencing platform. The E-Gel® SizeSelect™ system,

  • Dramatically reduces the time required to construct libraries
  • Provides tight, consistent size recovery of fragments <1Kb in size

The Smartest Way to Gel-Purify DNA for Cloning Reactions

E-Gel® SizeSelect™ gels are the smartest way to gel-purify DNA fragments <1Kb in size for downstream cloning reactions. Purified DNA can be used directly in any cloning reaction without the need for additional purification kits. The proprietary stain incorporated in the E-Gel® SizeSelect™ gels is five times more sensitive than ethidium bromide, allowing maximal retrieval of DNA bands. Avoid the personal hazards of UV exposure by using the Safe Imager™ Real-Time Transilluminator for visualization migrating DNA bands. The blue light produced by the Safe Imager™ Real-Time Transilluminator, ensures integrity of the sample DNA which in turn maximizes cloning efficiency.

Choose E-Gel® SizeSelect™ gels for cloning fragments <1Kb and enjoy:

  • Optimal cloning results—minimal DNA damage and maximal yield
  • Time savings—have DNA ready for cloning in minutes
  • Enhanced personal safety—avoid exposure to harmful UV radiation

 

 Figure 3. Cloning efficiency with E-Gel® SizeSelect™ Gels and the Safe Imager® blue light transilluminator. PCR-generated inserts of 3 different molecular weights were cloned into pCR® 2.1 TOPO® vector using manufacturer’s protocol. Prior to cloning, the fragments were purified from either E-Gel® incorporated with ethidium bromide, or E-Gel® SizeSelect™ gels. Fragments were retrieved from E-Gels with ethidium bromide by excision using a razor blade followed by column purification. Reactions were performed in triplicates and data are presented as average values. Standard deviations are represented as error bars.