RNA Century™ Markers (100 – 1,000 bases): RNA Century™ Markers comprise a set of 5 in vitro–generated transcripts of 100, 200, 300, 400, and 500 bases. RNA Century™-Plus Markers comprise a set of 7 in vitro–generated transcripts of 100, 200, 300, 400, 500, 750, and 1,000 bases. The Century™ Markers and Century™ Marker Templates are ideal for use in isotopic and nonisotopic ribonuclease protection assays and when single-stranded RNA size standards are needed for denaturing gel electrophoresis. 

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Century™ Marker Templates

The Century™ Marker Templates contain mixtures of linearized plasmids ready for use as templates in in vitro transcription reactions for synthesis of labeled RNA molecular size standards. When transcribed with T7 RNA polymerase, Century™ Marker Templates produce 5 RNA transcripts of 100, 200, 300, 400, and 500 bases. Century™-Plus Marker Templates produce 7 RNA transcripts of 100, 200, 300, 400, 500, 750, and 1,000 bases.

 

  Figure 1. (A) RNA Century™ Markers and RNA Century™-Plus Markers (2 µg), along with an RNA transcript, were electrophoresed on an 8 M urea/5% polyacrylamide gel and stained with ethidium bromide.

Ambion® Century™ Marker Selection Guide

DNA Ladders Base Pair (bp) Range
 RNA Century™ Markers  100–500 bases
 RNA Century™-Plus Markers  100–1,000 bases
 Century™ Marker Templates  100–500 bases
 Century™-Plus Marker Templates  100–1,000 bases