Nucleic Acid Quantitation
|Regardless of throughput, we've got a fluorescence-based quantification kit for your DNA and RNA samples. Utilizing simple mix-and-read protocols and incorporating fluorescence-based detection reagents, these assays are designed for accuracy and convenience.
Which assay kit is right for me?
|1–20 samples||20–2,000 samples||> 2,000 samples
|Qubit® assay kits for use with the Qubit® 3.0 Fluorometer||Quant-iT™ assay kits and reagents for use with microplate readers *||Quant-iT™ bulk reagents and kits|
Qubit® assay kits for 1–20 samples
The Qubit® assay kits for use with the Qubit® 3.0 Fluorometer are designed for quantification of smaller numbers of samples (~1–20 samples, read in 0.2 mL tubes). Get rapid results with as little as 2 minutes' assay incubation and ~5 seconds' sample read time. Based on target-specific fluorescent dyes, Qubit® assays offer accurate quantification data, even when samples contain common contaminants.
Performance of the Qubit® dsDNA HS Assay. The Qubit® dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.
Quant-iT™ assay kits
Quant-iT™ assay kits utilize advanced fluorophores that become fluorescent upon binding to DNA, RNA, or proteins; the fluorescence intensity of the resulting complex depends directly on the amount of the target molecule in the sample.
Quant-iT™ assay kits for 20–2,000 samples
The Quant-iT™ Kits, 1,000 assay size, provide the ultimate in convenience for DNA, RNA, or protein quantitation in microplates. These are the best choice for quantitating 20–2,000 samples at a time.
Quantitation using Quant-iT™ Assay Kits. (Left) The Quant-iT™ dsDNA Assay Kit, High Sensitivity has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double the amount shown. The inset shows the sensitivity of the assay for DNA. (Center) The Quant-iT™ RNA Assay Kit has a linear detection range of 5–100 ng and is selective for RNA, even in the presence of an equal mass of DNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double what is stated. (Right) The Quant-iT™ Protein Assay Kit has a detection range of 0.25–5 μg. This graph shows the signal generated from nine different proteins over the stated detection range. The protein-to-protein variability at the 3 μg point for all nine proteins assayed is 12%.
Quant-iT™ bulk reagents and kits for >20,000 samples
These Quant-iT™ products provide the maximum amount of dye in packaging that is amenable to highly automated quantitation environments. These are the best choice for quantitating more than 2,000 samples at a time.
These products are available in either concentrated Quant-iT™ dyes or Quant-iT™ kits with buffer and a standard included.
Figure 2. Nucleic acid quantitation using Quant-iT™ Reagents. (Left) The fluorescence signal from binding of the Quant-iT™ PicoGreen® dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/mL calf thymus DNA. (Center) The Quant-iT™ RiboGreen® RNA Assay provides linear quantitation from 20 ng/mL to 1 μg/mL RNA using a 400-fold dilution of the dye; the inset shows the results for 1.0 ng/mL to 50 ng/mL RNA obtained using a 4,000-fold dilution of the dye. (Right) Quantitation of a synthetic 24-mer using the Quant-iT™ OliGreen™ ssDNA Reagent is linear over at least four orders of magnitude; the inset shows the results obtained using 0–2.0 ng/mL of oligo.