The protocol begins with total RNA purified from culture, tissues, or blood using a method of choice (e.g., PureLink™ RNA Mini Kit, TRIzol® Reagent)
The total RNA is then hybridized with Locked Nucleic Acid (LNA) probes specific for abundant ribosomal RNA molecules.
Next, the unwanted rRNA are separated from the RiboMinus™- enriched complexes using RiboMinus™ Magnetic beads.
The RiboMinus™-enriched RNA sample is then concentrated using ethanol precipitation or a silica spin column step.
The enriched RNA fraction is now ready for downstream processing (e.g., microarray, RNA-Seq).