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MicroRNAs (miRNAs) are an important class of small RNAs that regulate protein translation. Much effort has gone into understanding how, when, and where miRNAs are produced and function in cells, tissues, and organisms. Each miRNA is thought to regulate multiple genes, and since hundreds of miRNAs are predicted to be present in higher eukaryotes the potential regulatory circuitry afforded by miRNAs is enormous. Some scientists believe that the number of miRNAs could exceed 1,000. Cloning and sequencing have been used to identify most of the known mammalian miRNAs. Isolating the mature miRNA fraction is the first critical step in cloning these small regulatory molecules, and Ambion® scientists have developed the tools you’ll need for your miRNA cloning experiments.

flashPAGE™ System

The Ambion® flashPAGE™ fractionator system is a fast, easy, and consistent method for PAGE purification of small nucleic acids. The innovative flashPAGE™ fractionator system enables:

  • Ultrafast PAGE purification of nucleic acids—with standard gel run times of only ~12 minutes
  • An easy, streamlined protocol
  • Consistent, reliable results with precisely formulated, ready-to-use gels and reagents

 

Learn more about the flashPAGE™ System

mirVana™ miRNA Isolation Kit

Efficient Recovery of Small RNA
This kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10mers—from virtually all cell and tissue types. The mirVana™ miRNA Isolation Kit enables

  • Efficient isolation of small RNA–containing total RNA
  • Enrich for small RNA <200 nt to increase sensitivity in downstream analyses
  • Ideal for miRNA, siRNA, shRNA, and snRNA analysis

 


Learn more about the mirVana™ miRNA Isolation Kit

Ordering Information

Sku Name Size Price Qty
AM1560 mirVana™ miRNA Isolation Kit, with phenol 40 preps USD 288.00