Next-generation sequencing has transformed gene expression profiling and other RNA expression analysis studies. With an increasing number of researchers employing next-generation sequencing, and with the evolution of sequencing platforms, it is essential to use robust and streamlined methods for sample preparation. The best next-generation sequencing data begin with optimal samples.

Learn more about RNA & Transcriptome Sequencing

RNA Sequencing Overview

Next-generation sequencing studies require robust and streamlined methods for sample preparation. The best next-generation sequencing data begin with optimal samples. Click the tabs above to learn more and see all available products.

RNA Isolation

Although many methods are available for purifying RNA from any particular source, obtaining RNA of the highest purity is the best assurance that the downstream manipulations will be successful. Choose from various formats, including PureLink®, mirVana™, RecoverAll™, and MagMAX™ kits to isolate total RNA, then enrich for the subpopulation of interest.

Simple, reliable, rapid method 30 min isolation from most samples HTP format, ideal for stringent applications Best for next-generation sequencing of mRNA High-throughput purification of FFPE samples
RNA types isolated for sequencingLarge RNA molecules only
(mRNA and rRNA)
Small & large RNA molecules
(microRNA, tRNA, mRNA, rRNA)
Small & large RNA molecules
(microRNA, tRNA, mRNA, rRNA)
mRNA onlytotal RNA, microRNA, gDNA
Isolation methodFast, convenient silica column Highest purity and convenience; includes organic extraction and silica column Scalable, flexible format for highest purity; includes organic extraction and magnetic beadsFast magnetic bead capture directly from microsized samples Xylene-free, scalable, flexible format with magnetic beads
Prep time

20 min

30 min

<1 hr

15 min3 hr
(for 96 preps)
Amount of starting material

10 mg to 100 mg of tissue
Up to 5 x 107 cells

Up to 100 mg of tissue
Up to 1 x 107 cells 
Up to 100 mg of tissue
Up to 1 x 107 cells 
Up to 500 ng purified Total RNA2 x 10 micron FFPE sections
Prep size50 preps40 preps96 preps2 mL oligo dT beads (sufficient for 100 mRNA preps) 96 preps
Kit price$225$225-266$373$587$768
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RNA Stabilization

The RNA-Seq workflow begins with RNA stabilization to ensure the maintenance of undegraded total RNA. The second key element of the workflow involves using efficient methods for RNA purification and preparation to provide high-quality material.

Ambion® RNAlater® Tissue Collection: RNA Stabilization Solution is an aqueous, nontoxic tissue-storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA.

  • Single reagent immediately inactivates RNases and stabilizes RNA within tissues or cells
  • No need to freeze samples in liquid nitrogen or rush samples back to the lab freezer
  • Eliminates the need to freeze and grind most tissue samples
  • Perfect for tissue collection “in the field”
  • RNA is stable for 1 day at 37°C, 1 week at 25°C, 1 month at 4°C, or indefinitely at –20°C
  • Compatible with many RNA isolation procedures, including most RNA isolation kits

Learn more about RNAlater® solution

Ordering Information


Cat. No.

Product

Quantity

AM7020

RNAlater®

100 mL

AM7024

RNAlater®

250 mL

AM7021

RNAlater®

500 mL

AM7022

RNAlater® (1.5 mL tube)

50 tubes

AM7023

RNAlater® (5 mL bottle)

20 bottles

RNA Enrichment

To maximize the effectiveness of sequencing reagents, it is often necessary to enrich the sample for specific regions or targets of interest. This enrichment helps eliminate nonspecific artifacts, making it possible to better analyze smaller changes in genomes or transcriptomes and less abundant sequences. Ambion® RiboMinus™ technology is designed to enrich the whole spectrum of RNA transcripts, regardless of their polyadenylation status or the presence of a 5' cap, by selectively depleting rRNA. The RiboMinus™ method removes the vast majority of rRNA (up to 99.9%) to allow greater interrogation of the less abundant transcripts.

Learn more about RiboMinus™ technology



A novel and efficient method utilizing Locked Nucleic Acids (LNA®) to remove up to 99% rRNA A novel and efficient method utilizing Locked Nucleic Acids (LNA®) to remove up to 99% rRNA
PlatformDiagnosticDiagnostic
Starting material1–10 µg total RNA
100–500 ng total RNA (low-input protocol)
1–10 µg total RNA
RiboMinus RNA yield1–2 µg from 10 µg human total RNA
~100 ng from 500 ng human total RNA (low-input protocol)
1–2 µg from 10 µg human total RNA
Probe specificityEukaryoticPlant
Cat. No.A1083708 - 8 prepsA1083808 - 8 preps
Kit price$499.49$499.49
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RNA Library Construction

Library construction, template preparation, and sequencing are at the heart of the sequencing workflow. Utilization of sequencing controls and methods for data analysis complete the workflow and provide useful annotated data. Generate cDNA by reverse transcription from adaptors ligated to the ends of RNA, then amplify the cDNA using primers complementary to the adaptors, and purify it.

Prepare representative cDNA libraries for strand-specific RNA sequencing A Great Solution for NGS The Ultimate Solution for NGS
PlatformIon Torrent™ PGM™SOLiD® 5500 AnalyzerIllumina
RNA input typerRNA-depleted total RNA, poly(A) RNA, or miRNATotal RNA, rRNA-depleted total RNA, poly(A) RNA, or miRNATotal RNA, rRNA-depleted total RNA, poly(A) RNA, or miRNA
RNA input range

Small RNA: total RNA containing 1–100 ng of miRNA

Whole transcriptome:
100 ng total RNA (~1 ng poly(A) or ~25 ng rRNA-RNA

Small RNA: total RNA containing 1–100 ng of miRNA

Whole transcriptome:
100 ng poly(A), 200 ng

Small RNA: total RNA containing 1–100 ng of miRNA

Whole transcriptome:
100 ng poly(A) or 200 ng rRNA-depleted total RNA, or 200 ng total RNA

Barcodes available

Yes

96No
Cat. No.4475936 - 12 reaction kit4445374 - 12 reaction kit4454073 - 12 reaction kit
Kit price$1,200 $1,433 $1,650
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RNA Library Controls

Library construction, template preparation, and sequencing are at the heart of the sequencing workflow. Utilization of sequencing controls and methods for data analysis complete the workflow and provide useful annotated data.

Learn more about ERCC Controls



2 tubes containing preformulated mixes of the same 92 RNA transcripts but in different ratios, for measuring dynamic range, assessing bias, and confirming expression fold changes Single tube containing a preformulated mix of 92 RNA transcripts for measuring dynamic range and assessing bias
Top SellerTop seller
Compatible sample typesEukaryotic total RNA, rRNA-depleted total RNA, or poly(A) RNA
Eukaryotic total RNA, rRNA-depleted total RNA, or poly(A) RNA
Dynamic range6 logs6 logs
Volume10 µL (2 tubes)10 µL
Number of reactionsSpike-in controls diluted based on sample type and concentrationSpike-in controls diluted based on sample type and concentration
Kit price$2,000 $1,000
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RNA Barcoding

Sets of 16 barcodes are available for use with the SOLiD® Total RNA-Seq Kit or the SOLiD® SAGE™ Kit with Barcoding Adaptor Module. Barcodes cost-effectively increase the number of samples that can be analyzed in a single sequencing run. Barcodes assign a unique identifier to templated beads made from a single library. Once assigned, the RNA libraries can be pooled and sequenced.

Cat. No.

Product

4475485

Ion Xpress™ RNA-Seq Barcode 1-16 Kit

4427046

SOLiD® RNA Barcoding Kit, Module 1-16

4453189

SOLiD® RNA Barcoding Kit, Module 17-32

4453191

SOLiD® RNA Barcoding Kit, Module 33-48

4456501

SOLiD® RNA Barcoding Kit, Module 49-64

4456502

SOLiD® RNA Barcoding Kit, Module 65-80

4456503

SOLiD® RNA Barcoding Kit, Module 81-96

4461565

SOLiD® RNA Barcoding Kit, Module 1-48

4461566

SOLiD® RNA Barcoding Kit, Module 49-96

4461567

SOLiD® RNA Barcoding Kit, Module 1-96

RNA Sequencing

No matter which platform you choose to suit your needs, Life Technologies continues to support the evolving needs of this field with gold-standard instrumentation, innovative next-generation technology, optimized best-in-class reagents, consumables, analysis software, applications and world-class technical support.


Next-Generation (SOLiD®)
Sequence right here, right now—minimizing wait time and costs for wasted reagents.
 Semiconductor (Ion Torrent) Sequencing
Sequence from your benchtop with Ion Torrent™ revolutionary "on-chip" technology.
 
Capillary Electrophoresis Sequencing
The gold standard in sequencing and a fast, flexible platform for analysis.
 RNA & Transcriptome Sequencing
Choose the correct technology for your specific RNA application.