RNA Structure/Function Studies
RNA structure is thought to play a central role in many cellular processes, including transcription initiation, elongation and termination, mRNA splicing, and retroviral infection of eukaryotic cells. Elucidating the mechanistic aspects of these intricate processes will require detailed understanding of the underlying RNA structure. RNA molecules usually possess a variety of single-stranded and double-stranded regions that give rise to complex three-dimensional structures. These structures are involved in the molecule's interactions with other nucleic acids, proteins, and small molecules. We have developed an extensive portfolio of Ambion® products for the synthesis and modification of RNA in order to further understand the role of RNA structure.
Tools for RNA Structure/Function Research
In Vitro Transcription Kits
1. Moine H, Ehresmann B, Ehresmann C, and Romby P. (1998) Probing RNA structure and function in solution. In: Simons RW and Grunberg-Manago M (editors). RNA Structure and Function, Cold Spring Harbor Laboratory Press. p. 77-115.
2. Knapp G. (1989) Enzymatic approaches to probing of RNA secondary and tertiary structure. Methods Enzymol 180:192-212. Abstract
3. Krol A and Carbon P. (1989) A guide for probing native small nuclear RNA and ribonucleoprotein structures. Methods Enzymol 180:212-227. Abstract
4. Tinoco I Jr, and Bustamante C. (1999) How RNA folds. J Mol Biol 293(2):271-281. Abstract
5. Doudna J A. (2000) Structural genomics of RNA. Nature Struct Biol 7 Suppl:954-956. Abstract
- TechNotes 8(5): Products for RNA Structure/Function Analysis: Biochemical grade RNases and modified rNTPs for structural characterization of RNA
- TechNotes 9(4): End Labeling RNA for Structural Analysis: Tips for radiolabeling your RNA on either the 5' or 3' end for subsequent nuclease digestion or footprinting analysis.
- Technical Bulletin: Methods for Enzymatic Nonisotopic Labeling of RNA by In Vitro Transcription