showcase.par.71603.image.0.0.1

Small amounts of ribonucleases (RNases) can sometimes co-purify with isolated RNA and compromise downstream applications. Such contamination can also be introduced via tips, tubes, and other reagents used in procedures. RNase inhibitors are commonly used as a precautionary measure in enzymatic manipulations of RNA to inhibit and control for such contaminants. Life Technologies offers several nuclease inhibitors: SUPERase•In™ RNase Inhibitor, RNaseOUT™ Recombinant Ribonuclease Inhibitor, ANTI-RNase, RNAsecure™ Reagent and Ribonuclease Inhibitor.

Product Selection Guide

Function Product Description
Nuclease InhibitorSUPERase•In™ RNase Inhibitor (20 U⁄μL)
  • Robust protein-based RNase inhibitor
  • Inhibits RNases A, B, C, 1 and T1
  • Active at up to 65°C and from pH 5.5 to 8.5
  • No DTT requirements
  • Requires no DTT, but is functional in DTT concentrations as high as 200 mM DTT
  • Retains activity in multiple freeze-thaw cycles
RNaseOUT™ Recombinant Ribonuclease Inhibitor
  • Noncompetitive, recombinant, protein-based RNase inhibitor
  • Inhibits RNase A, B, and C
ANTI-RNase (15–30 U⁄μL)
  • Protein-based ribonuclease inhibitor
  • Inactivates RNases A, B, and C
  • Does not release active RNase in absence of DTT or other reducing agent
  • Ideal for use in in vitro transcription and translation, cDNA synthesis PCR, RT-PCR, RNA isolation procedures, and preparation of RNase-free antibodies
RNAsecure™ Reagent
  • A nonenzymatic reagent that irreversibly inactivates RNases in solutions that
    • Cannot be treated with DEPC
    • Cannot be autoclaved
    • Contain enzymatic reactions prior to adding the enzyme
  • Does not require post-treatment autoclaving
  • Reheating after initial treatment reactivates the RNase-destroying agent to minimize new contamination
RNase Inhibitor
  • Recombinant ribonuclease inhibitor
  • Inhibits RNases A, B, and C
  • Does not inhibit RNase I, T1, T2, H, U1, U2, or CL3
  • Requires a minimum of 1 mM DTT
  • Effective pH range: 5.0–8.0
RNase Inhibitor (Cloned) 40 U⁄μL
  • Cloned version of RNase inhibitor

Ordering Information