Bleaching. Ultraviolet treatment. Autoclaving. To the average molecular biologist, eliminating nuclease contamination, particularly ribonuclease (RNase) contamination, is a constant and somewhat annoying challenge. RNA is an especially sensitive and difficult molecule to work with, as it is readily degraded. Therefore, it is essential that labware, instruments, and surfaces involved in the purification of RNA are free from RNases. Even trace quantities of RNases can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results with qRT-PCR.
All surfaces, including benchtops, pipettes, glassware, and benchtop instruments, should be treated with a surface decontamination agent. Life Technologies offers several surface decontamination products that are proven effective at eliminating RNase contamination from lab surfaces—RNaseZap® Solution, RNaseZap® Wipes, ElectroZap™ Solution, and RNase AWAY® Reagent.
Product Selection Guide
|Surface Decontamination||RNaseZap® Solution||
|RNase AWAY® Reagent||
Surface Decontamination Support Materials
- How to Maintain an RNase-Free Lab - Technical Bulletin #180
- Nuclease and Protease Testing - Technical Bulletin #166
- RNase and DEPC Treatment: Fact or Laboratory Myth? - Technical Bulletin #178
- Working With RNA - Technical Bulletin #159
- Combat RNase Contamination in the Lab - TechNotes 11(2): We offer a complete selection of Ambion® products to help prevent, detect, and eliminate RNase contamination in molecular biology experiments
- Detect RNases Before They Ruin Your Experiment - TechNotes 8(2): New 30-minute test for verifying that common lab solutions are RNase-free.
- Measuring RNase Activity - A Real-time Kinetic Analysis - TechNotes 8(4): Detect nuclease and standardize enzyme activity.
- RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control - TechNotes 12(3): A comparison of total RNase activities in eight different mouse tissues, with recommendations for the best RNA isolation methods.
- SUPERase•In™: The Right Choice for Protecting Your RNA - TechNotes 8(2): New ribonuclease inhibitor that inhibits a broader range of RNases over a broader range of conditions than traditional RNase inhibitors.
- Which Water to Use? - TechNotes 11(4): Don't overlook the water used in the experiment when trying to pinpoint the source of RNase contamination.
- Your Data: RNase A Molecules are Used to Study the Structure of Amyloid-Like Fibrils: A summary of recently published data on protein refolding during the formation of amyloid fibrils and the retention of native protein function demonstrated through the use of our Ambion® RNaseAlert® technology.