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Ribonucleic acid (RNA) is an especially sensitive and difficult molecule to work with because it is readily degraded. To help ensure success, steps must be taken to minimize nuclease contamination in RNA purification laboratories. Even trace quantities of RNase can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results with qRT-PCR.

Life Technologies offers several Ambion® products in the RNaseZAP® family—RNaseZap® Solution, RNaseZap® Wipes, and ElectroZap™ Solution—that collectively provide a comprehensive approach to help ensure that work surfaces, pipettors, and equipment are RNase-free.

RNAseZap® products offer:

  • Better RNase A decontamination than competitive products (Figure 1)
  • Excellent decontamination of dried-on RNase A (Figure 2)

Product Selection Guide

Function Product Description
Surface Decontamination
RNaseZap® Solution Glass and plastic surface decontaminant
RNaseZap® Wipes Glass and plastic surface decontaminant in towelette form
ElectroZap™ Solution Noncorrosive cleaning solution, formulated to decontaminate electrode surfaces
 

 

Figure 1. Comparison of Available Cleaning Products  for the Removal of RNase Contamination. Mouse liver total RNA (2 µg) was added to microcentrifuge tubes precontaminated with RNase A and then cleaned with  different commercially available RNase decontamination products:  "a", "b", or RNaseZap® solution. The tubes were then rinsed twice with water prior to the addition of the RNA  in 20 µL of TE. The samples were incubated at 37°C for 30 min. Ten microliters of each sample was resolved on a 1.5% agarose gel (TAE) and visualized on a UV light box.

 

Figure 2. Removal of Dried-on RNase Contamination. A 5 µg sample of RNase A was vacuum-dried onto the bottom of two microfuge tubes. One tube was then rinsed with water, and one tube was rinsed with RNaseZap® solution. Two micrograms of total RNA was added to each of the microcentrifuge tubes, incubated at 37°C for 30 min, and assessed on a 1.5% agarose gel.

Ordering Information

Sku Name Size Price Qty
AM9780 RNaseZap® RNase Decontamination Solution 250 mL USD 63.50
AM9780M RNaseZap® RNase Decontamination Solution with Manual 250 mL USD 61.25
AM9782 RNaseZap® RNase Decontamination Solution 6 x 250 mL USD 284.00
AM9782M RNaseZap® RNase Decontamination Solution with Manual 6 x 250 mL USD 284.00
AM9784 RNaseZap® RNase Decontamination Solution 4 L USD 560.00
AM9786 RNaseZap® RNase Decontamination Wipes 100 sheets USD 75.50
AM9786M RNaseZap® RNase Decontamination Wipes with Manual 100 sheets USD 75.50
AM9788 RNaseZap® RNase Decontamination Wipes Refill 300 sheets USD 173.00
AM9788M RNaseZap® RNase Decontamination Wipes Refill with Manual 300 sheets USD 173.00
AM9785 ElectroZap™ Electrode Decontamination Solution 250 mL USD 63.50

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