MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) modulate gene expression. Along with several other classes of small noncoding RNAs (e.g. snRNA, snoRNA and piRNA), they have tremendous impact on a variety of biological processes.

While most traditional RNA isolation methods are not very efficient at recovering these smaller RNAs, several kits developed by the Ambion® RNA experts offer quantitative recovery of small RNAs from a variety of sample types.

Which microRNA & small RNA Isolation Kit if Right for You?

  Rapid purification for enrichment of small RNAs only 30 min isolation from most samples Purification for enrichment of specific small RNAs HTP, pure, concentrated microRNAs Complete kit, sample-to-qPCR from cells
Top Selleryes   
RNA types isolatedSmall RNAs onlySmall & large RNA moleculesSmall RNAs onlySmall & large RNA moleculesSmall & large RNA molecules
Prep time20 minutes30 minutes75 minutes<1 hour10 minutes
Compatible sample typesFresh or frozen tissue, tissue stored in RNAlater, cellsMost sample typesFresh or frozen tissue, blood, cellsFresh or frozen tissue, tissue stored in RNAlater, cellsCells
Isolation methodFast, convenient silica columnHighest purity and convenience; includes both organic extraction and silica column10-50 μL blood-related samples; 5 x 104 cultured cells or 1-10 mg tissueScalable, flexible format for highest purity; includes organic extraction and magnetic beadsCells-to-CT™ direct lysis, no purification required
Reverse transcription reagents includedNoNoNoNoTaqMan® MicroRNA Reverse Transcription Kit
qPCR Master mix includedNoNoNoNoTaqMan®
Kit size25 preps40 preps40 Preps96 preps100 lysis reactions
- plus -
reagents for 200 qRT-PCR assays
Kit Price$175.88$225-266.00$340.00$373.00$1,356.00
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Ambion Publications

  • Johnson CD, Esquela-Kerscher A, Stefani G, Byrom M, Kelnar K, Ovcharenko D, Wilson M, Wang X, Shelton J, Shingara J, Chin L, Brown D, Slack FJ. The let-7 MicroRNA Represses Cell Proliferation Pathways in Human Cells. Cancer Res. 2007 Aug 15;67(16):7713-22. [Abstract]
  • Shingara J, Keiger K, Shelton J, Laosinchai-Wolf W, Powers P, Conrad R, Brown D, Labourier E. An optimized isolation and labeling platform for accurate microRNA expression profiling.RNA. 2005 Jul 25; [Epub ahead of print] [Abstract]
  • Johnson SM, Grosshans H, Shingara J, Byrom M, Jarvis R, Cheng A, Labourier E, Reinert KL, Brown D, Slack FJ. RAS is regulated by the let-7 microRNA family. Cell. 2005 Mar 11;120(5):635-47. [Abstract]
  • Cheng AM, Byrom MW, Shelton J, Ford LP. Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis. Nucleic Acids Res. 2005 Mar 1;33(4):1290-7. [Abstract]

Ambion Publications

  • Johnson CD, Esquela-Kerscher A, Stefani G, Byrom M, Kelnar K, Ovcharenko D, Wilson M, Wang X, Shelton J, Shingara J, Chin L, Brown D, Slack FJ. The let-7 MicroRNA Represses Cell Proliferation Pathways in Human Cells. Cancer Res. 2007 Aug 15;67(16):7713-22. [Abstract]
  • Shingara J, Keiger K, Shelton J, Laosinchai-Wolf W, Powers P, Conrad R, Brown D, Labourier E. An optimized isolation and labeling platform for accurate microRNA expression profiling.RNA. 2005 Jul 25; [Epub ahead of print] [Abstract]
  • Johnson SM, Grosshans H, Shingara J, Byrom M, Jarvis R, Cheng A, Labourier E, Reinert KL, Brown D, Slack FJ. RAS is regulated by the let-7 microRNA family. Cell. 2005 Mar 11;120(5):635-47. [Abstract]
  • Cheng AM, Byrom MW, Shelton J, Ford LP. Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis. Nucleic Acids Res. 2005 Mar 1;33(4):1290-7. [Abstract]