Starting with high quality, pure, and intact total RNA is critical to many experiments, (e.g. RT-PCR, qRT-PCR, array analysis, Northern blots, nuclease protection assays and RNA sequencing). The table below will help you choose the right Ambion® product to purify total RNA from your specific sample type and sample size.

Which Total RNA Purification Kit is Right for You?

  Gold standard for highly pure, intact RNA High-quality RNA in less than 20 minutes High-throughput purification of RNA and DNA Complete, no purification system for qRT-PCR results
RecommendationsMost Cited, Top Seller  Best for qPCR
Isolation methodHigh purity organic extraction (requires alcohol precipitation)Fast, convenient silica column Scalable, flexible format with magnetic beadsChemical lysis (time to qRT-PCR results)
Prep time~1 hr< 20 minutes~45 minutes10 minutes
Compatible sample typesMost sample types, particularly more difficult to lyseCells and tissuesCells, blood, plantsCells
Amount of starting material100 mg of tissue or 107 cells (requires 1 mL reagent)Up to 200 mg tissue, 5x106 to 1x108 cellsUp to 100 mg tissue, Up to 5x106 cells1–100,000 cells
Reverse transcription reagents included   yes
qPCR Master mix included   yes
High throughput compatible yes
(plate format available)
yesyes
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Organic Extraction & Purification

Organic solvent extraction offers tried-and-true performance, and is ideal for the most stringent applications when combined with silica-membrane filtration or magnetic bead purification. TRIzol® Reagent offers:

  • Broadest sample type compatibility
  • Robust method
  • Good for difficult samples (e.g. high lipid tissue) and scalable sample input
  • Wide acceptance as the gold standard

Learn more about TRIzol® Reagent

Magnetic Bead Purification

Magnetic particles offer solution-phase binding and washing kinetics to facilitate RNA purification and improved sample handling, including automation, ideal for higher throughput processing. MagMAX™ Magnetic Beads offer:

  • Flexible and scalable throughput
  • Amenable to automation for higher throughput
  • Better removal of inhibitors and low elution volume resulting in more concentrated RNA

Learn more about MagMAX™ Magnetic Beads

Ambion® Brand RNA Essentials

Ambion® brand products, are designed to minimize nuclease contamination and ensure experimental success in less-than-ideal conditions. These products are manufactured under the most stringent quality specifications and include:

  • RNA purification kits
  • Specimen collection and RNA stabilization solutions
  • Nuclease inhibitors and decontaminants
  • Nuclease-free buffers and plastics
  • RNA ladders
  • Northern blot reagents

Learn more about Ambion® brand products

Silica-membrane Purification

Silica-membrane filters allow fast and easy RNA purification resulting in pure RNA that can be used in most applications. PureLink™ Purification columns offer:

  • A fast and easy method suitable for most downstream uses
  • Wide sample type compatibility
  • Familiar spin column format commonly used for low-med throughput

Learn more about PureLink™ Purification columns

Technical Resources