The RiboPure™ RNA Isolation Kits combine a phenol and guanidine thiocyanate solution , a robust lysis and denaturing reagent, with a glass-fiber filter purification method to provide RNA of high yield and quality. The phenol and guanidine thiocyanate reagent is a monophasic solution that reliably and rapidly lyses cells and simultaneously inactivates nucleases. The use of glass-fiber filters for RNA purification after phase separation simplifies the process and eliminates impurities. The result is exceptionally pure RNA (including microRNA and other small RNAs), free of proteins, organic solvents, and other contaminants. The RNA contains minimal genomic DNA contamination and is ideal for critical downstream applications, including RNA amplification for microarray analysis, real-time RT-PCR, and northern blotting.
The RiboPure™ RNA Isolation Kit
is suitable for isolation of RNA from a broad range of biological samples, including 5-100 mg fresh, frozen, and RNAlater
® Solution-treated tissues, and 0.1-20 million mammalian cultured cells. RiboPure™ Kits are also available for RNA isolation from blood
, and bacteria
. The protocol is fast, simple, reliable, and can be completed in less than 30 minutes.