Influenza detection requires several distinct steps, including isolation of the viral RNA, reverse transcription of the viral RNA to cDNA, and realtime PCR-based amplification and detection of known subtype sequences. Life Technologies supplies high-performing products for each step in this process.

Products for Influenza Research Include:

Research Tools for Detection

Step 1. Isolate Viral RNA
PureLink™ Viral RNA/DNA Mini Kit
Isolates high-quality and highly concentrated viral DNA and RNA from cell-free samples such as serum, plasma, and cell culture—ideal for downstream applications, such as real-time PCR. Available in both spin-column and 96-well plate formats for fast and easy isolation
iPrep™ PureLink™ Virus Kit
The iPrep™ PureLink™ Virus Kit allows for a simple yet standardized procedure for isolating viral RNA from a range of cell-free samples using bead-based technology.

 

Step 2. Reverse Transcribe & Amplify cDNA
SuperScript® III Platinum® One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format.
Step 3: Detect Influenza Subtype
Primers & Probes for H1N1 Virus Detection

New Product - Pandemic H1N1/09 Assay Set v 2.0

This product employs assay designs licensed from the Centers for Disease Control and Prevention (CDC). Includes the primers and probes required to run the CDC Influenza 2009 A(H1N1) pdm Real-time rRT-PCR Detection Protocol.

Catalog No. 4456927 - not available for sale in the US or Japan

Contact your local sales representative

 

New Product - Pandemic H1N1/09 Assay Controls v 2.0

Includes 5 controls (4 positive controls (1 for each assay) and 1 negative template control).

Catalog No. 4456926 - not available for sale in the US and Japan.

Contact your local sales representative

Kits for Influenza A Virus Screening
Custom TaqMan® Influenza A Detection Kit 2.0 (not for sale in U.S. or Japan)
For initial influenza type A screening, this kit provides a consistent, rapid, and unparalleled easy-to-use standardized procedure for detecting the presence of Influenza A viral nucleic acid targets derived from a variety of samples. These kits contain RT-PCR reagents, a positive control, and TaqMan® probes for detecting Influenza A.
Step 4: Perform Real-Time PCR
7500 Fast DX Real-Time PCR Instrument
The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, with SDS v1.4 Software, is a 96-well, 5-color real-time PCR instrument available for in vitro diagnostic use.
7500 Fast Real-Time PCR System
Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
Accommodate varying sample throughputs
7500 Fast Service Contract
7500 Fast Qualification (IQOQ) and Instrument Performance Verification Services.