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BioTek’s Synergy™ H4 and Synergy™ 2 multi-mode microplate readers have received the LanthaScreen® Certified Plus designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before Using LanthaScreen® Terbium and Europium Assays

Test Your Plate Reader Set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

Synergy™ H4 Hybrid Multi-Mode Microplate Reader


 

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide

Application Note View View View View View View
Poster - View View View View View
Excitation Filter (18 mm)  340/30 400/30 340/30 360/40 530/25  400/30 
Emission 1 Filter (18 mm)  620/10 460/40 495/10 485/20 590/35 460/40 
Emission 2 Filter (18 mm)  665/8 528/20 520/25 NA 590/35  528/20 
Dichroic Mirror  400 NA 400 400  570 435 

 

Synergy™ 2 Multi-Mode Microplate Reader


 

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide

Application Note View View View View View View
Poster - View View View View View
Excitation Filter (18 mm) 340/30 400/30 340/30 360/40 530/25  400/30 
Emission 1 Filter (18 mm) 620/10 460/40 495/10 485/20 590/35 460/40 
Emission 2 Filter (18 mm) 665/8 528/20 520/25 NA 590/35  528/20 
Dichroic Mirror 400 NA 400 400 570 435

Synergy™ HT Multi-Mode Microplate Reader

 

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide

Reader not compatible

Reader not tested

Reader not compatible

Reader not tested

Reader not compatible

Reader not tested

Application Note - View - - - View
Poster - - - - - -
Excitation Filter (18 mm) - 400/30 - 340/30 - 400/30 
Emission 1 Filter (18 mm) - 460/40 - 485/20 - 460/40 
Emission 2 Filter (18 mm) - 528/20 - NA - 528/20 

FLx800™ Fluorescence Microplate Reader

 

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide

Reader not compatible

Reader not compatible

Reader not compatible

Application Note - - - - - -
Poster - View - View - View
Excitation Filter (18 mm) - 400/30 - 360/40 - 400/30 
Emission 1 Filter (18 mm) - 460/40 - 485/20 - 460/40 
Emission 2 Filter (18 mm) - 528/20 - NA - 528/20 



* Filters (diameter) shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm). Contact BioTek Instruments for information on ordering filters and dichroic mirrors suitable with the microplate readers listed above. 

Synergy™ and FLx800™ are trademarks of BioTek Instruments.