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The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen® Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

SpectraMax® Paradigm® Microplate Detection Platform


 

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide
Application Note
Poster - -
Excitation Filter   406/15     532/25  
Emission 1 Filter   465/35     595/35   
Emission 2 Filter   535/25     595/35   
Dichroic Mirror   508        
Optic Module   GeneBLAzer®     FP rhodamine   

 

SpectraMax® M5/M5e Microplate Reader
& FlexStation® 3 Benchtop Multi-Mode Microplate Reader


 
 

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide

Reader not
compatible

Application Note - - - - - -
Poster - - - - - -
Excitation 332/12 410/12 332/12 360/12   410/12
Emission 1 620/12 458/12 488/12 485/12   458/12
Emission 2 666/12 522/12 518/12     522/12
Dichroic Mirror 590/630 435/495 420 Auto   435/495

FilterMax™ F5 Multi-Mode Microplate Reader


 

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide Reader not compatible Reader not compatible Reader not compatible
Application Note
Poster - -
Excitation Filter   405/30    360/35    405/30
Emission 1 Filter   465/35    485/20    465/35
Emission 2 Filter   535/25        535/25
Dichroic Mirror            

 

Analyst® Multimode Reader


     

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide

-

-

 

-

-

-

Application Note - - - - - -
Poster - - - - - -
Excitation Filter (25 mm) 340/30 (PV00225) 405/20 340/30 (PV00225) 340/30 (PV00225) 530/25 405/20
Emission 1 Filter (25 mm) 620/7.5 460/40 495/10 (PV00325) 495/10 (PV00325) 580/10 460/40
Emission 2 Filter (25 mm) 665/7.5 530/25 520/25 (PV00325)   580/10 530/25
Dichroic Mirror 380 or
400
425 or
50/50
380 or
400
380, 400 or 50/50 561 425 or
50/50

 

* Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above.

** Some compatible filter sets are available for purchase from Life Technologies.  Click on selected filter sets for more information.

Analyst®, SpectraMax® and FlexStation® are registered trademarks of Molecular Devices.