Many of the PerkinElmer plate readers including the Envision®, ViewLux® and VICTOR™ are compatible with Life Technologies assay technologies for TR-FRET, FRET, Fluorescence Intensity (kinetic), and Fluorescence Polarization.

Before using LanthaScreen® Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

EnVision® Multilabel Reader

 
 

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide      

Application Note - - - - - -
Poster - - Lab Auto 08 - - -
Excitation Filter (15 mm) 340/30
(PV00215)
400/25 340/30
(PV00215)
340/30 (PV00215) 531/25 FP 400/25
Emission 1 Filter (15 mm) 615/8.5 460/25 495/10
(PV00315)
495/10 (PV00315) 579/25 FP P-pol 460/25
Emission 2 Filter (15 mm) 665/7.5 535/25 520/25
(PV00315)
NA 579 FP S-pol 535/25
Dichroic Mirror D400/630 TRF Dual or D400 TRF D425/495 BLA Dual or D425 BLA; 50/50 General Dual or 50/50 General D400/630 TRF Dual or D400 TRF; D400/505 LanthaScreen® Dual 50/50 General or D400 TRF D555fp/D595 Dual or D555fp D425/495 BLA Dual or D425 BLA; 50/50 General Dual or 50/50 General

 

VICTOR™ Multilabel Plate Readers

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide Reader compatible but settings not validated Reader not
compatible
  Reader compatible but settings not validated Reader not
compatible
Reader not
tested
Application Note - - - - - -
Poster - - - - - -
Excitation Filter (15 mm) 340/60
(built-in)
- 340/60
(built-in)
340/30
(PV00215)
- NA
Emission 1 Filter (25 mm) 616/8.5 - 495/10
(PV00325)
495/10
(PV00325)
- NA
Emission 2 Filter (25 mm) 665/7.5 - 520/25
(PV00325)
NA - NA

 


 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) N/A N/A
Setup Guide Reader compatible but settings not validated Reader not
tested
  Reader compatible but settings not validated Reader not
tested
Reader not
tested
Application Note - - - - - -
Poster - - Lab Auto 08 - - -
Excitation Filter (60 mm) 340/60 NA 340/60 340/60 NA NA
Emission 1 Filter (60 mm) 616/8 NA 495/10
(Chroma)
480/20 NA NA
Emission 2 Filter (60 mm) 671/7.8 NA 520/20 NA NA NA
Dichroic Mirror 400 NA 400 400 NA NA



* Filters (diameter) shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact PerkinElmer for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above

** Some compatible filter sets are available for purchase from Life Technologies.
  Click on selected filter sets for more information.

Envision® and ViewLux® are registered trademarks and Victor™ is a trademark of PerkinElmer.