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The Varioskan® Flash from Thermo Scientific has received the LanthaScreen® Certified designation from Life Technologies which ensures that this reader is validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen® Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

Varioskan® Flash Multimode Reader




 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Omnia® Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FI (kinetic) FP FRET
Setup Guide

Reader not
compatible

Application Note  - - -
Poster  - - - - - -
Excitation 340/12  409/12 332/12 360/12  - 400/12 
Emission 1 615/12  460/12 488/12 485/12  - 460/12 
Emission 2 665/12  530/12 518/12 NA - 530/12 



* Optics shown as center wavelength/bandwidth (nm); Contact Thermo Scientific for information on detection modalities suitable with the microplate reader listed above.                    
                    
Varioskan® is a registered trademark of Thermo Scientific.