Fura-2 Calcium Indicator
- Fluorescent Ca2+ indicator allows accurate measurement of intracellular calcium concentrations
- Ratiometric readout minimizes the effects of photobleaching, leakage, uneven loading, and varying cell thicknesses in mixed populations, delivering more robust and reproducible results
- Particularly well-suited for ratio-imaging microscopy
- Well-established in literature, with many citations in numerous cell lines
At low concentrations of the indicator, 340/380 nm excitation ratio for fura-2 allows accurate measurements of the intracellular Ca2+ concentration (Figure 1). Measuring by ratio considerably reduces the effects of uneven dye loading, leakage of dye, and photobleaching, as well as problems associated with measuring Ca2+ in cells of unequal thickness. Measurements of fura-2 fluorescence can usually be made over a period of an hour without significant loss of fluorescence resulting from either leakage or bleaching. In addition, fura-2 is bright enough to permit measurements at intracellular concentrations of dye unlikely to cause significant Ca2+ buffering or damping of Ca2+ transients.
||Figure 1: Fluorescence excitation spectra of fura-2 (F1200, F6799) in solutions containing 0–39.8 µM free Ca2+.|