A LanthaScreen® Eu Kinase Binding Assay requires three primary components: a kinase protein, a kinase tracer, and a europium-labeled antibody. The table below lists the recommended components to build a binding assay for your kinase of interest. The general components required to run the binding assay are:
- A kinase protein—This table lists kinases that are sold by Invitrogen and which have been tested and validated with this assay format.
- A kinase tracer—There are six kinase tracers available which are suitable for over 200 kinase assays. This table lists the Invitrogen recommended tracer for specific kinases, based on our validation experiments.
- A europium-labeled antibody—The assays listed in the table below have been validated with one of our Invitrogen europium antibodies. This table lists the Invitrogen recommended eu-antibody for your kinase of interest.
- An assay buffer—or most LanthaScreen® Eu Kinase Binding Assays, the recommended buffer is 5X Kinase Buffer A, please review the protocol for your kinase for further information.
The kinase binding assay format can also be applied to your target of interest, regardless of the reagent vendor or target. Contact our specialized Technical Support Specialists for more information.