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Measure Kinase Activity in Real Time

  • Easy protocol - just mix, then read fluorescence
  • Kinetic format - suitable for mechanistic studies
  • Study substrate inhibitors
  • Fast—get results in under an hour

Omnia® Kinase Assays utilize a fluorescence peptide substrate-based technology for the rapid, homogenous and sensitive detection of enzymatic activity. Results are measured under optimal kinetics, physiological (mM) ATP, and in real time without use of radioactive tracers or specialized equipment. Increases in kinase activity result in a corresponding increase in fluorescence. The kinetic format of the assay also corrects for false positives (compound auto fluorescence) and false negatives (quenching), minimizing interference. The assay can be used to select for both ATP-competitive and ATP non-competitive (allosteric) inhibitors.

Learn more about the Omnia® Kinase Principle >

Kinase (112 total) Best Kit
AKT1ST1
AKT2ST1
AKT3ST1
AMPKST23
ARK5ST31
AURORA AST16
AURORA BST16
AURORA CST16
BRSK1 
CAMK1D (CAMKIδ)ST23
CAMK2A (CAMKIIα)ST23
CAMK2D (CAMKIIδ)ST23
CDC42BPA (MRCK/DMPK)ST18
CDC42BPB (MRCK/DMPK)ST18
CDK1/CyclinBST7
CDK1/CyclinEST7
CDK2/CyclinAST7
CDK2/CyclinEST7
CDK4/CyclinD1 
CDK5/p25NCKST7
CDK5/p35ST7
CDK6/CyclinD1 
CHEK1 (CHK1)ST11
CHEK2 (CHK2)ST3
CLK2ST15
CLK3ST15
CSNK1A1 (CK1)ST24
CSNK1D (CKIδ)ST24
CSNK1E (CK1ε)ST24
CSNK1G1 (CK1γ1)ST24
CSNK1G2 (CK1γ2)ST24
CSNK1G3 (CK1γ3)ST24
CSNK2A1 (CK2α1)ST22
DAPK3ST1
DCAMKL2 
DYRK1A 
GSK3αST13
GSK3β (C-terminal His)ST13
IKKβ (IKK2)ST20
IKKεST24
MAP3K10 (MLK2)ST6
MAP3K11 (MLK3)ST8
MAP3K9 (MLK1)ST8
MAP4K2 (GCK)ST25
MAP4K4 (HGK)ST32
MAP4K5 (KHS1)ST26
MAPK1 (ERK2)ST17
MAPK10 (JNK3)ST10
MAPK11 (p38β)ST9
MAPK12 (p38γ)ST7
MAPK13 (p38δ) 
MAPK14 (p38α) 
MAPK3 (ERK1)ST17
MAPK8 (JNK1)ST10
MAPKAP-K2 (MK2)ST3
MAPKAP-K3ST3
MAPKAP-K5 (PRAK)ST3
MARK2ST31
MINK1ST8
MLK1ST8
MLK3ST8
NEK1 
NEK2 
NEK4 
NEK6ST27
NEK9 
PAK1ST5
PAK2ST5
PAK3ST5
PAK4ST5
PAK6ST5
PAK7ST5
PASKST6
PHKG1ST3
PHKG2ST3
PIM1ST26
PIM2ST14
PKAST5
PKCαST8
PKCβIST8
PKCβIIST8
PKCδST8
PKCεST8
PKCηST8
PKCγST8
PKCιST8
PKCθST8
PKCζST8
PKCμST8
PKN1 (PRK1)ST18
PRKCN (PKD3)ST8
PRKD2ST8
PRKG1ST5
PRKG2ST5
PRKXST5
ROCK1ST5
ROCK2ST5
RPS6KA1 (RSK1)ST6
RPS6KA2 (RSK3)ST6
RPS6KA3 (RSK2)ST6
RPS6KA5 (MSK1)ST1
RPS6KA6 (RSK4)ST6
RPS6KB1 (p70S6K)ST6
SGK (SGK1)ST1
SGK2ST4
SGKL (SGK3)ST4
STK22B (TSSK2)ST31
STK22D (TSSK1) 
STK3 (MST2) 
STK4 (MST1) 
TAOK2 (TAO1) 
TBK1 (IKK-ε)ST12

 

Kinase (77 total) Best Kit
ABL1Y6
ABL1 E255KY6
ABL1 G250EY6
ABL1 T315IY6
ABL1 Y253FY6
ABLl2 (ARG)Y6
ALKY4
AXLY11
BLKY5
BMXY5
BTKY5
CSF-1R (FMS)Y9
EGFR (ErbB1)Y12
EGFR T790MY12
EGFR L858RY10
EGFR L861QY12
EGFR1Y7
EPHA1Y6
EPHA2Y5
EPHA4Y12
EPHA5 
EPHA8Y12
EPHB1Y6
EPHB2Y6
EPHB3Y6
EPHB4Y5
FERY5
FGFR1Y12
FGFR2Y11
FGFR3Y11
FGFR3 K650EY12
FGFR4Y10
FGRY5
FLT1 (VEGFR1)Y8
FLT3 CDY5
FLT3 D835YY5
FLT4 (VEGFR3)Y8
FRKY5
FYNY5
HCKY5
IGF-1RY4
IR (Insulin Receptor)Y4
IRR (Insulin-related receptor)Y4
ITKY6
JAK2Y11
JAK2 JH1 JH2Y12
JAK2 JH1 JH2 V617FY12
JAK3Y7
KDR (VEGFR2)Y8
KITY9
KIT T670IY12
LCKY5
LYN AY5
LYN BY5
MERTK (cMER)Y6
MET (HGFR)Y12
MET (HGFR) M1250TY9
MST1RY8
MUSKY1
NTRK1 (TRKA)Y8
NTRK2 (TRKB)Y5
PDGFRA (PDGFRα)Y9
PDGFRA D824VY9
PDGFRB (PDGFRβ)Y14
PTK2 (FAK)Y3
RETY12
RET V804LY8
RET Y791FY11
ROS1Y4
SRCY2
SRCN2 
SRMSY12
SYKY7
TEK (tie2)Y8
TYRO3 (RSE)Y5
YESY5
ZAP70Y8

Table 1. Ordering information for reagents

Reagents Cat. No.
Omnia® Ser/Thr Reaction BufferKB001A
Omnia® Tyr reaction BufferKB002A
Omnia® ATP SolutionAS001A

Omnia® Custom and Bulk Substrate Services

Should you not find your substrate of interest, Invitrogen also offers custom services for the generation of Omnia® peptide substrates. Omnia® substrates can be successfully produced for a wide variety of kinases. We also can provide milligram quantities of lyophilized substrate for your large screening projects.

Please contact us at discoverysciences@invitrogen.com for more information about these services or to request a quotation.