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BacMam reagents can be used in a variety of mammalian cell types.  View the cell types that have been transduced using BacMam technology and learn how to optimize conditions for your cell type.

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Suitable for a Wide Range of Cell Types

Cell types successfully transduced with BacMam reagents include numerous transformed cell lines (e.g. U2-OS, HEK 293, and HeLa). Beyond transformed cell lines BacMam transduction of many primary cells (e.g., fibroblasts, hepatocytes, cardiovascular cells and epithelial cells) and stem cells (e.g. neuronal and mesenchymal) have been shown to be highly efficient and generally without adverse affects, making this platform a very promising gene delivery platform technology. However, cells of hematopoietic origin show consistently poor transduction by BacMam technology, and are not recommended for use with the current technology. See Table 1 for a generalized comparison of the transduction efficiencies of BacMam 1.0 and BacMam 2.0 across different cell types. Gene expression can be increased by the use of an enhancer (HDAC inhibitor).

Table 1. Comparison of BacMam 1.0 and BacMam 2.0 across different cell types. Gene expression can be increased by using the BacMam Enhancer.

 
BacMam 1.0
No enhancer
BacMam 1.0
With enhancer
BacMam 2.0
No enhancer
BacMam 2.0
With enhancer
Cell lines
+/–
++
++
++
Primary cells
+
++
++
++
Stem cells
++
++
++
Neurons
++
++
Immortalized T-cells
+/–
+
Primary T-cells and B-cells

     =  transduction efficiency <10%
+/–  =  transduction efficiency ~10%
+     =  transduction efficiency ~50%
++   =  transduction efficiency >50%