Chromatin immunoprecipitation (ChIP) experiments examine histone modifications and genomic DNA sequences bound to specific regulatory proteins. In ChIP, protein-DNA complexes are crosslinked in vivo, immunoprecipitated, purified, and amplified for gene- and promoter-specific analysis of known targets or for the identification of new target sequences. In a related microarray-based method, ChIP-on-Chip, the immunoprecipitated DNA is labeled and hybridized to high-resolution microarrays.
Invitrogen offers a variety of products for chromatin immunoprecipitation, including protein A and G reagents, antibodies, and PCR and qPCR reagents.
Invitrogen offers a number of products for the ChIP-on-Chip workflow, including reagents for immunoprecipitation, PCR, and microarray labeling and hybridization.
Chromatin Research Topics
- Overview of chromatin
- Histone modifications and histone variants
- Chromatin remodeling and gene regulation