Purification Column Changes
We want to inform you that the current purification columns included in this kit are no longer available and we have decided to make improvements in the purification step rather than discontinue this product.
The new purification module contains the same columns that we use in the newer BioPrime® Total Labeling kits that include Alexa Fluor® dyes. Though we are changing the columns we are not changing the catalog number for the kit. We have seen no difference in performance other than an improvement in the removal of unincorporated dye which does reduce the background signal on the arrays.
Purelink™ columns reduce background
Comparisons of Macherey Nagel (BioPrime® Array CGH Genomic Labeling System – lanes A,C) and PureLink™ (BioPrime® Array CGH Genomic Labeling System – lanes B,D) purified products shows that the majority of the free nucleotides are removed with PureLink™ purifications. In addition PureLink™ captures more high molecular weight reaction products. The labeling data further indicated that there is no purification bias for labeled and unlabeled nucleotides, as the Degree of Labeling was not effected by two different purification methods.