ArcturusXT™ LCM Instrument

The ArcturusXT™ microdissection system is a unique combination of infrared (IR) laser-enabled laser capture microdissection (LCM) and ultraviolet (UV) laser cutting in one platform. The open, modular design of the ArcturusXT™ system offers unparalleled research flexibility and versatility. The combination of Arcturus® LCM instruments and Arcturus® microgenomics reagents gives you the only complete solution for microgenomics research.

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Key Features

  • Consolidated design—precise IR laser capture microdissection and UV laser cutting in one single instrument
  • Fast functioning—rapid isolation of specific cells in four simple steps
  • Open, modular platform—flexible to suit numerous research needs
  • Easy, intuitive operation—simplified workflow through streamlined interface
  • Flexible sample prep—wide range of slide types and sample preparations supported

A Unique Offering of Laser Capture and Laser Cutting Microdissection

The solid-state IR laser—exclusive to the ArcturusXT™ microdissection system—delivers a gentle capture technique, which preserves biomolecular integrity and is ideal for single cells or a small number of cells. The solid-state UV laser offers unprecedented speed and precision, well-suited for microdissecting dense tissue structures and for rapidly capturing large numbers of cells.

Rapidly Isolate Pure Cell Populations for Microgenomic Analysis

Laser capture microdissection allows for rapid isolation of specific cells in four simple steps. After locating the cells of interest, the CapSure® LCM Cap is placed over the target area. Pulsing the ArcturusXT™ System laser through the cap activates the film on the cap to adhere to the target cells. Lifting the cap removes the target cells now attached to the cap. The isolated cells  are now available for extraction of biomolecules.

Modular Flexibility to Fit Your Needs

The ArcturusXT™ microdissection instrument is modularized to suit any dynamic research need (Figure 1). The system utilizes a Nikon Eclipse Ti-E inverted research microscope with a variety of options available at the time of purchase or post-purchase. Each instrument has IR-enabled LCM, an interactive pen-display monitor, and a trackball actuated stage for easy and ergonomic navigation. Additional modules that may be added include LED brightfield illumination or high-intensity halogen illumination, enabling phase contrast and differential Interference contrast (DIC) microscopy applications.

The ArcturusXT™ system may be purchased with or without UV laser cutting and attenuable fluorescence. A wide range of objectives are available, including a 2x –100x dry objective and a 100x oil-immersion objective. For ultimate image quality and analysis, a high-resolution megapixel camera may be added.

Complete LCM System Figure 1. Complete LCM system: Harvest single cells or tens of thousands of cells in seconds.

Simplified Workflow Through Streamlined User Interface

The simple, intuitive user interface places the focus on research (Figure 2). From loading of samples to extraction of biomolecules, the uncomplicated workflow maximizes experimental efficiency.

 Figure 2. Operation made easy. The simple, intuitive user interface places the focus on research. From sample loading to extraction of biomolecules, the uncomplicated workflow maximizes experimental efficiency.

Ultimate Flexibility in Sample Source and Preparation

The unique combination of IR laser capture and UV laser cutting permits the use of many slide types including glass, glass membrane, and framed membrane slides for contact or non-contact microdissection. In addition, a variety of specimen preparations may be used (Figures 3, 4, and 5).

 Figure 3. Superior optics for subcellular microdissection. Versatile enough to handle many sample types, the ArcturusXT™ system optics can be used to visualize your samples for microgenomics research.

 
Figure 4. Rapidly isolate pure cell populations for microgenomics research. Different tissue types are shown before and after laser capture microdissection to identify cells of interest, isolate them, and prepare them for microgenomics analysis.

 
Figure 5. Isolate different cell types for microgenomics research. Arcturus® labeling kits can be used to identify cellular components of interest for further analysis.

Instrument Specifications

MicroscopeSTANDARD: Nikon Eclipse® Ti-E microscope base
STANDARD: Standard microscope operation outside of LCM operating software
OPTIONAL: Binoculars, CFI 10x eyepieces
LasersSTANDARD: Infrared (IR) Capture Laser: Solid-state, near-IR (810 nm)
OPTIONAL: Basic UV Cutting Laser: Solid-state, passive Q-switched, diode-pumped (355 nm)
OPTIONAL: Enhanced UV Cutting Laser: Solid-state, diode-pumped Q-switched (345 nm) with adjustable laser current (0 –100%) and pulse frequency (10 – 5000 Hz)
IlluminationSTANDARD: High-intensity LED illumination system
OPTIONAL: Nikon Diascopic Illumination Tower for contrast imaging (100 W halogen lamp)
ObjectivesSTANDARD: 2x, 10x, and 40x Nikon CFI60 objectives
OPTIONAL: 4x, 20x, 60x, 100x dry and 100x oil Nikon CFI60 objectives
StageSTANDARD: Motorized, trackball-actuated in X and Y axes with 1 μm precision
STANDARD: Stage insert for three 75 mm x 25 mm slides
OPTIONAL: Stage insert for large-format slides;
two positions, adjustable to 75 mm x 25 mm, 75 mm x 38 mm, 75 mm x 50 mm
OPTIONAL: Stage insert for Petri dish: 50 mm x 7 mm
Contrast MethodsSTANDARD: Bright-field
OPTIONAL: Phase contrast (PhL, Ph1, Ph2)
OPTIONAL: Differential interference contrast (DIC), Senarmont method (rotating polarizer)
Dimensions30" (D) x 22" (W) x 29" (H)
Work Surface Required36" x 72" (92 cm x 183 cm), 33" (80 cm) vertical clearance
Power100 – 240 VAC, 50 – 60 Hz; 600 W
Operating Temperature18 – 30°C
Operating Humidity<60% relative humidity (non-condensating)
Altitude For use up to 6,600 ft (2,000 m)
Computer Requirements
  • 3.0 GHz Intel Core 4 (min)
  • 4 GB RAM (min)
  • 2 x 500 GB hard drives-RAID 1
  • DVD±RW drive
  • Windows 7 Pro
  • 1280 x 1024 dpi resolution monitor
  • Interactive pen display monitor
Other FeaturesFor a full list of features and specifications, see the ArcturusXT™ Product Brochure



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The ArcturusXT™ LCM Instrument is for research use only, and is not intended for animal or human therapeutic or diagnostic use.