Digital PCR is a new approach to nucleic acid detection and quantification. It offers a different method for absolute quantification and rare allele detection relative to conventional real-time quantitative PCR. Digital PCR works by partitioning a sample into many individual real-time PCR reactions; some portion of these reactions contain the target molecule (positive) while others do not (negative). Following PCR analysis, the fraction of negative reactions is used to generate an absolute count of the number of target molecules in the sample, without reference to standards or endogenous controls.
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Digital PCR for absolute quantification
Digital PCR is quantitative PCR method that can be used for absolute target quantification.
|Applications||Advantages of digital PCR|
Digital PCR on the QuantStudio™ 3D Digital PCR System
The QuantStudio™ 3D Digital PCR System enables digital PCR experiments in a manner previously unattainable.
- Absolute data with high precision and sensitivity—detect and count individual molecules.
- Simple workflow—obtain digital data with a chip-based workflow. Just load and go.
- Affordable platform—start doing digital PCR immediately with lower cost barriers.
- Compatible—use your existing TaqMan® real-time PCR assays for a digital result.
- Sealed system—limit contaminants with a sealed chip and no exposed sample transfer steps.
- Wide dynamic range—achieve up to 5 logs of dynamic range.
- Intuitive—see data directly on instrument touchscreen and easily transfer to a computer for analysis with AnalysisSuite™ Digital PCR Software.
Regulatory Status: For Research Use Only. Not for use in diagnostic procedures.