Absolute quantification, using sealed-chip technology, for a reliable method and precise, sensitive data.
Differential Gene Expression Using Digital PCR
Detect low-fold changes in gene expression
Real-time PCR is commonly used to detect differential gene expression; however, this approach is generally limited to detecting changes that vary by two-fold or more. For some studies, detection of expression changes less than two-fold may be required. Furthermore, it is often necessary to express differential gene expression with respect to a reference gene, such as a housekeeping gene like actin.
With the ability to achieve highly precise measurements of ±10% or better, digital PCR is capable of resolving changes of two-fold or less.
In addition, the ability of digital PCR to determine absolute quantification of a transcript obviates the need for a reference gene. Like real-time PCR, digital PCR requires the conversion of RNA to cDNA. Since the efficiency of conversion is important to experimental sensitivity, we offer the High-Capacity cDNA Reverse Transcription Kit, which seamlessly integrates into your digital PCR gene expression workflow.
Poster: Precise and Accurate Determination of MicroRNA Precursors by Digital PCR
Product Bulletin: QuantStudio™ 3D Digital PCR System
User Guide: QuantStudio™ 3D Digital PCR System
Digital PCR Experiment Design Guide
For Research Use Only. Not for use in diagnostic procedures.