GMO detection with dPCR

Sensitive detection of plant mutations

Understanding the genetics behind plant phenotypes is a complex and laborious process. In addition, due to labelling requirements in many countries, sensitive tests for genetically modified organisms (GMO) are becoming more necessary. Real-time PCR has been the gold standard for measuring plant gene expression; however, as the concentration of potential targets decreases among high backgrounds of wild-type material, and when inhibitors are present, real-time PCR can prove to be a challenging method for quantifying plant mutations and for GMO detection. 

Approaches such as digital PCR allow researchers to decipher the expression of wild type versus mutant genes, measuringspecific alleles (SNPs) and detecting new rare mutations. Digital PCR on the QuantStudio™ 3D Digital PCR System enables nucleic acid detection and quantification that is ideal for GMO detection and measuring plant mutations because it provides an absolute number for targets in a sample. In addition, digital PCR doesn’t rely on standards and offers a high tolerance for the many PCR inhibitors that are routinely encountered in plant samples.

Literature
QuantStudio™ 3D Digital PCR System

QuantStudio™ 3D Digital PCR System

Absolute quantification, using sealed-chip technology, for a reliable method and precise, sensitive data.

Request a quote

Ordering information

Sku Name Size Price Qty
4428234 GeneAmp® PCR System 9700, Dual Flat Block 1 instrument Contact Us  
4485513 QuantStudio™ 3D Digital PCR Chip Adapter Kit for Flat Block Thermal Cycler 1 kit USD 46.35
4485507 QuantStudio™ 3D Digital PCR Chips 12 chips USD 102.00
4482710 QuantStudio™ 3D Digital PCR Master Mix 1.5 mL USD 140.00
4485718 QuantStudio™ 3D Digital PCR Master Mix 5 mL USD 410.00
4489084 QuantStudio™ 3D Digital PCR Instrument with Power Cord 1 instrument USD 30,900.00