Elongase® is a mixture of Taq DNA Polymerase (Recombinant) and Pyrococcus species GB-D thermostable DNA polymerase. The ratio of the two thermostable polymerases is such that the processivity and activity of Taq and the 3'-5'exonuclease of the Pyrococcus species GB-D polymerase allow for the amplification of fragments up to 30 kb (up to 30 kb with lambda sequence and up to 20 kb for a single copy sequence in genomic DNA) and the greater amplification of smaller fragments. Amplification using Taq DNA polymerase is generally limited to DNA template less than 5 kb. This limitation is believed to be largely due to the inability of Taq DNA Polymerase, which lacks proofreading 3'-5' exonuclease activity to correct nucleotide misincorporations and continue primer elongation. Including a small amount of proofreading enzyme edits the nascent strand to allow subsequent polymerization by Taq.

Components Elongase® Enzyme Mix Elongase® Enzyme Mix
Catalog Number1048001010480028
Enzyme Mix100500
5X Buffer A1.0 ml5 x 1.0 ml
5X Buffer B1.0 ml5 x 1.0 ml

Ordering Information

Catalog # Name Size List Price (USD) Qty
10480010 Elongase® Enzyme Mix 100 reactions 189.00
10480028 Elongase® Enzyme Mix 500 reactions 836.00
10790020 PCR SuperMix High Fidelity 100 reactions 233.00