• Amplify a broad range of targets (<3 kb) which contain high GC, high AT, homopolymer runs, and more
  • Superior yield allows you to do more with your PCR amplicons
  • Decreased false positive results with reduced bacterial background
  • Reproducible results from a trusted source

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AmpliTaq® 360 DNA Polymerase Amplifies a Broad Range of Targets

See how AmpliTaq® 360 DNA Polymerase compares to other suppliers below:

Figure 1. Comparison of AmpliTaq® 360 DNA Polymerase with other DNA Polymerases.

Panel A shows data produced with AmpliTaq® 360 DNA Polymerase while Panel B shows the same amplicons amplified using the Roche Taq DNA Polymerase. PCR reactions were performed using 1 ng of template DNA and 1.25 units of enzyme in each 50 uL reaction. Annealing was uniform across the selected targets. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 basepairs in length with an average length of 553. Each reaction was performed in duplicate. The high GC target reactions included 2–10 uL of 360 GC Enhancer depending on the target used.

Figure 2. Comparison of AmpliTaq® 360 DNA Polymerase with other DNA Polymerases.

Panel A shows data produced with AmpliTaq® 360 DNA Polymerase while Panel B shows the same amplicons amplified using the Stratagene Taq2000 DNA Polymerase. PCR reactions were performed using 1 ng of template DNA and 1.25 units of enzyme in each 50 uL reaction. Annealing was uniform across the selected targets. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 basepairs in length with an average length of 553. Each reaction was performed in duplicate. The high GC target reactions included 2–10 uL of 360 GC Enhancer depending on the target used.

Figure 3. Comparison of AmpliTaq® 360 DNA Polymerase with other DNA Polymerases.

Panel A shows data produced with AmpliTaq® 360 DNA Polymerase while Panel B shows the same amplicons amplified using the New England Biolabs Taq DNA Polymerase. PCR reactions were performed using 1 ng of template DNA and 1.25 units of enzyme in each 50 uL reaction. Annealing was uniform across the selected targets. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 basepairs in length with an average length of 553. Each reaction was performed in duplicate. The high GC target reactions included 2–10 uL of 360 GC Enhancer depending on the target used.

Figure 4. Comparison of AmpliTaq® 360 DNA Polymerase with other DNA Polymerases.

Panel A shows data produced with AmpliTaq® 360 DNA Polymerase while Panel B shows the same amplicons amplified using the Sigma RedTaq DNA Polymerase. PCR reactions were performed using 1 ng of template DNA and 1.25 units of enzyme in each 50 uL reaction. Annealing was uniform across the selected targets. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 basepairs in length with an average length of 553. Each reaction was performed in duplicate. The high GC target reactions included 2–10 uL of 360 GC Enhancer depending on the target used.

Figure 5. Comparison of AmpliTaq® 360 DNA Polymerase with other DNA Polymerases.

Panel A shows data produced with AmpliTaq® 360 DNA Polymerase while Panel B shows the same amplicons amplified using the Promega GoTaq Green. PCR reactions were performed using 1 ng of template DNA and 1.25 units of enzyme in each 50 uL reaction. Annealing was uniform across the selected targets. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 basepairs in length with an average length of 553. Each reaction was performed in duplicate. The high GC target reactions included 2–10 uL of 360 GC Enhancer depending on the target used.

Specificity

AmpliTaq® 360 DNA Polymerase provides better specificity than other Taq reagents.

Summary of 2 replicates for each of 40 amplicons indicating the average specificity for each amplicon and the standard deviation. AmpliTaq Gold® 360 PCR reactions were performed using 1 ng of template DNA per reaction using cycling conditions according to the manufacturer’s recommendations.

Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1400 base pairs (bp) in length with an average length of 553 bp.

Data shown here compares AmpliTaq® 360 DNA Polymerase (blue) with New England BioLabs Taq Polymerase (light green), Roche Taq Polymerase (orange), Sigma RedTaq DNA Polymerase (light blue), Promega GoTaq Polymerase (green), and Strategene Taq2000 (red).

 

Figure 6: Specificity Comparison Between AmpliTaq® 360 DNA Polymerase and Various Suppliers.
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Sensitivity

AmpliTaq® 360 DNA Polymerase provides good sensitivity.

Amplification of the beta-actin gene was performed from 0 to 80 starting copies of DNA, using 2.0 mM MgCl2 and standard thermal cycling conditions with a 30 second extension time.

 
 Figure 7: Sensitivity of AmpliTaq® 360 DNA Polymerase for Detection of Low Copy Amplicons
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Long Target Amplification

AmpliTaq® 360 DNA Polymerase reproducibly amplifies long targets.

Plasmid amplicons up to 5,459 bp were efficiently and reproducibly amplified in duplicate reactions. Plasmid DNA was amplified using 2 mM MgCl2 and standard thermal cycling conditions with a 6 minute extension time.

 
 Figure 8: Amplification of Long Plasmid Targets
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Human Raji cell amplicons up to 3.8 kb pairs long were efficiently and reproducibly amplified in duplicate reactions. Human Raji cell DNA was amplified using 2 mM MgCl2 and standard thermal cycling conditions with a 4 minute extension time. 
 Figure 9: Amplification of Long Human DNA Targets
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Robust Sequencing

Optimized for sequencing applications, AmpliTaq® 360 provides amplicons that provide robust results.

Standard (A) and high GC % (B) amplicons were sequenced for both AmpliTaq® 360 DNA Polymerase and the original AmpliTaq®-generated PCR products.

10 ng of PCR products were used per sequencing reaction. Samples were cycle-sequenced using Big Dye® Terminator v3.1 and standard thermal cycling conditions. Big Dye XTerminator® was used to clean up the cycle sequencing reaction, and the samples were injected into the 3730xl sequencing instrument with POP-7 polymer.

 
 Figure 10: High-Quality Sequencing Data Obtained from AmpliTaq® 360 DNA Polymerase-Generated PCR Products.
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Ordering information

Sku Name Size Price Qty
4398813 AmpliTaq Gold® 360 DNA Polymerase 100 units USD 95.00
4398823 AmpliTaq Gold® 360 DNA Polymerase 250 units USD 238.00
4398833 AmpliTaq Gold® 360 DNA Polymerase 1,000 units USD 858.00
4398892 AmpliTaq Gold® 360 DNA Polymerase 1,500 units USD 1,158.00
4398894 AmpliTaq Gold® 360 DNA Polymerase 3,000 units USD 2,270.00
4398896 AmpliTaq Gold® 360 DNA Polymerase 5,000 units USD 3,570.00
4398898 AmpliTaq Gold® 360 DNA Polymerase 25 x 1,000 units USD 12,980.00
4398876 AmpliTaq Gold® 360 Master Mix 1 mL USD 112.00
4398881 AmpliTaq Gold® 360 Master Mix 5 mL USD 260.00
4398886 AmpliTaq Gold® 360 Master Mix 50 mL USD 1,570.00
4398901 AmpliTaq Gold® 360 Master Mix 10 x 5 mL USD 1,674.00