Instrument calibration

To ensure optimal performance of your thermal cycler or sequence detection system, Applied Biosystems strongly recommends that you regularly calibrate the system. Calibration schedules vary by instrument; refer to the table below for the types of calibration and appropriate intervals.

Instrument calibration intervals

Calibration TypeInstrumentInterval
ROI (Spatial)7300/7500/7500 Fast Real-time PCR Systems StepOne™ / StepOnePlus™ SystemsEvery 6 months or As needed to verify the instrument’s performance
Background7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR SystemsEvery month or Before performing a pure dye calibration or When installing an uncalibrated block
Pure dye spectra7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR SystemsEvery 6 months or When installing an uncalibrated block
Instrument verification7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR SystemsEvery 6 months or As needed to verify the instrument’s performance