What Can You Do with Real-Time PCR (qPCR)?
Real-time PCR, also known as qPCR, is used for many applications, including gene expression analysis, microRNA analysis, single nucleotide polymorphism (SNP) genotyping, copy number variation (CNV) analysis, and even protein analysis. This versatile technology can be used for both relative and absolute quantification methods. Real-time PCR applications are easy to implement using our wide selection of TaqMan® Assays and a comprehensive family of real-time PCR instruments. Learn more about the applications enabled by real-time PCR below.
Gene expression or messenger RNA (mRNA) analysis is the most commonly used application for real-time PCR. Researchers reverse-transcribe RNA, then use the cDNA produced as a template in real-time PCR to detect and quantitate expressed gene products.
From microRNA profiling to quantitation and detection of specific miRNAs, the TaqMan® microRNA Assay product line can help you quickly identify the microRNAs present in your sample and perform relative quantitation.
Real-time PCR instruments are commonly used for analysis of genomic variations. These techniques are not quantitative, and for genotyping using allele-specific TaqMan® Assays, data are collected after thermal cycling is complete (not in real time as the reaction progresses). Because these applications require a real-time PCR system, rather than a simple thermal cycler, in practice, they are typically considered real-time PCR applications.TaqMan® Drug Metabolism Genotyping Assays Reference Guide
Revolutionary TaqMan® Protein Assays combine the sensitive, specific protein-binding capabilities of antibodies with the superior relative quantitation capabilities of 5' nuclease real-time PCR.
Digital PCR is a new implementation of familiar real-time PCR technology that yields digital rather than analog data. It can provide absolute (rather than relative) quantitative target information with better sensitivity and precision than traditional real-time PCR, without reference to nucleic acid standards or controls.
For Research Use Only. Not for use in diagnostic procedures.