Gene Expression Analysis

Gene expression or messenger RNA (mRNA) analysis is the most commonly-used application for qPCR. Researchers reverse transcribe RNA, then use the cDNA produced as a template in qPCR reactions to detect and quantitate gene expression products.

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miRNA Analysis

From miRNA profiling, to quantitation and detection of specific miRNAs, the TaqMan® MicroRNA Assay product line can help you quickly identify the miRNAs present in your sample and perform relative quantitation.

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Digital PCR

Digital PCR is a new implementation of familiar qPCR technology that yields digital rather than analog data. It can provide absolute (rather than relative) quantitative target information with better sensitivity and precision than traditional qPCR, without reference to nucleic acid standards or controls.

Vogelstein B, Kinzler KW (1999) Digital PCR. Proc Natl Acad Sci USA, vol. 96 no. 169236–9241

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Genetic Variation Analysis

Real-time PCR instruments are commonly used for analysis of genomic variations. These techniques are not quantitative and for genotyping using allele-specific TaqMan® Assays, data are collected after thermal cycling is complete (not in real-time as the reaction progresses). Because these applications require a real-time PCR system, rather than a simple thermal cycler, in practice, they are typically considered real-time PCR applications.

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Protein Analysis using qPCR

Revolutionary TaqMan® Protein Assays combine the sensitive, specific protein-binding capabilities of antibodies with the superior relative quantitation capabilities of 5' nuclease real-time PCR.

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