OpenArray® Technology Overview
OpenArray® technology uses a microscope slide–sized plate with 3,072 through-holes. Each plate contains 48 subarrays, each with 64 through-holes. Each through-hole is 300 μm in diameter and 300 μm deep and is treated with hydrophilic and hydrophobic coatings. Reagents are retained in the through-holes via surface tension (see Figure). One OpenArray® plate can hold as many samples as can eight traditional 384-well plates.
Custom Plate Format
Custom OpenArray® plates for the QuantStudio™ 12K Flex system include a suite of 11 formats to provide flexible solutions for configuring your plates for analysis of gene expression, miRNA, or genotyping.
|Plate Format for Gene Expression Analysis||Samples/Plate||Min. Order||Samples/Min. Order|
|18 (3X) assays||48||10||480|
|Plate Format for Genotyping Analysis||Samples/Plate||Min. Order||Samples/Min. Order|
OpenArray® Workflow Overview with QuantStudio™ 12K Flex System
OpenArray® technology offers a very easy-to-use format. For most applications, assays are preloaded onto plates at our state-of-the-art manufacturing facility. To run the plates, simply mix your sample with master mix, load with the automated QuantStudio™ 12K Flex OpenArray® AccuFill™ System, cycle, and image (see Figure below).
OpenArray® technology helps streamline real-time PCR studies that use large numbers of samples, assays, or both, because a single QuantStudio™ 12K Flex OpenArray® plate is equivalent to running eight traditional 384-well plates. The system can run up to 4 QuantStudio™ 12K Flex OpenArray® plates simultaneously, allowing you to process up to 1,728 genotyping samples and 2,304 samples for gene expression, typically in an 8-hour day.
Enhance your genotyping throughput by thermal cycling offline using the Dual Flat Block GeneAmp® PCR System 9700 and process a total of more than 5,184 genotyping samples in one day. This system offers interchangeable blocks, allowing you to switch to standard multi-well plate formats as well.
Visit the OpenArray® application page to learn more of each application workflow and product.
Product Bulletin: OpenArray® Technology - get detailed information on applications using OpenArray technology and the QuantStudio™ 12K Flex system.
Regulatory Status: For Research Use Only. Not for human or animal therapeutic or diagnostic use.