OpenArray® technology uses a microscope slide–sized plate with 3,072 through-holes. Each plate contains 48 subarrays, each with 64 through-holes. Each through-hole is 300 μm in diameter and 300 μm deep and is treated with hydrophilic and hydrophobic coatings. Reagents are retained in the through-holes via surface tension (see Figure). One OpenArray® plate can hold as many samples as can eight traditional 384-well plates.

Custom Plate Format

Custom OpenArray® plates for the QuantStudio™ 12K Flex system include a suite of 11 formats to provide flexible solutions for configuring your plates for analysis of gene expression, miRNA, or genotyping.

Plate Format for Gene Expression Analysis Samples/Plate Min. Order Samples/Min. Order
18 (3X) assays4810480
56 assays4810480
112 assays2410240
168 assays1610160
224 assays1210120

Plate Format for Genotyping Analysis Samples/Plate Min. Order Samples/Min. Order
16 assays144101,440
32 assays9610960
64 assays4820960
128 assays2440960
192 assays1660960
256 assays1280960

OpenArray® Workflow Overview with QuantStudio™ 12K Flex System

OpenArray® technology offers a very easy-to-use format. For most applications, assays are preloaded onto plates at our state-of-the-art manufacturing facility. To run the plates, simply mix your sample with master mix, load with the automated QuantStudio™ 12K Flex OpenArray® AccuFill™ System, cycle, and image (see Figure below).

1 Select assays or panels
step 1

  • Select Assays and OpenArray® Plate formats for custom plates.
  • Select predesigned OpenArray® Pathways Panels.
  • Select OpenArray® Digital PCR Plates.

2 Add samples & master mix
step 2

For gene expression and genotyping applications, mix cDNA or DNA samples with master mix in 384-well sample plates. For digital PCR applications, mix assays, samples, and master mix in 384-well sample plates.

3 Load samples

step 3

Load sample mixes onto a QuantStudio™ 12K Flex OpenArray® plate with the QuantStudio™ 12K Flex AccuFill™ System.

4 Apply lid & fill
step 4

The QuantStudio™ 12K Flex OpenArray® plate encased in an alloy bottom for easy handling; simply apply the adhesive lid and fill case with immersion fluid.

5 Cycle, image, analyze data
step 5

Easily run up to 4 QuantStudio™ 12K Flex OpenArray® plates for any application on the QuantStudio™ 12K Flex instrument in standalone mode.

 

OpenArray® technology helps streamline real-time PCR studies that use large numbers of samples, assays, or both, because a single QuantStudio™ 12K Flex OpenArray® plate is equivalent to running eight traditional 384-well plates. The system can run up to 4 QuantStudio™ 12K Flex OpenArray® plates simultaneously, allowing you to process up to 1,728 genotyping samples and 2,304 samples for gene expression, typically in an 8-hour day.

Enhance your genotyping throughput by thermal cycling offline using the Dual Flat Block GeneAmp® PCR System 9700 and process a total of more than 5,184 genotyping samples in one day. This system offers interchangeable blocks, allowing you to switch to standard multi-well plate formats as well.

Visit the OpenArray® application page to learn more of each application workflow and product.