SNP Genotyping Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block
TaqMan® OpenArray® technology enables you to achieve the highest sample throughput for mid-density genotyping. In a single work day, 36 TaqMan® OpenArray® Genotyping Plates can be run on the OpenArray® Real-Time PCR Platform to generate data for >110,000 genotypes.
High-Throughput Results with Ease
The QuantStudio™ 12K Flex Real-Time PCR System and QuantStudio™ 12K Flex OpenArray® AccuFill™ System help enable you to process thousands of samples per day without robotics.
Each QuantStudio™ 12K Flex OpenArray® plate, encased in an alloy bottom for easy handling, comes preloaded with the assays of your choice—just add sample and master mix, cycle and image.
Best Experimental Flexibility
Choose any combination of pre-designed and custom TaqMan® SNP Genotyping Assays and the sample/SNP format that best meets your project needs.
Reliable and economical
Uses gold standard TaqMan® MGB probe chemistry in a nanofluidic design that reduces reagent usage
No oligo setup fee or hidden costs.
Experimental workflow: find the products you need for every step
Isolate and purify genomic DNA.
Products for Step 1: Prepare Sample
MagMAX™ DNA Multi-Sample Kit
Select or design TaqMan® probes using the TaqMan® OpenArray® Plate Configurator or Custom TaqMan® Assay Design Tool respectively. QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with TaqMan® OpenArray® Master Mix in TaqMan® OpenArray® 384-well sample plate.
TaqMan® OpenArray® Genotyping Plates
|Configure a Genotyping Plate
Configure TaqMan® OpenArray® Genotyping Plates using pre-designed assays and/or existing custom assay designs from your workspace
Design Your Own Custom Plate
Configure TaqMan® OpenArray® Genotyping Plates using the TaqMan® Custom Assay Design Tool
Load sample mixes onto QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plate using the QuantStudio™ 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. You can then perform on-line cycling on the QuantStudio 12K Flex system. To increase your throughput even more, as an option, you can amplify your on the Dual 96-well GeneAmp PCR System 9700 and perform an endpoint plate read using the QuantStudio 12K Flex system; TaqMan® SNP Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.
The data is plotted in an allelic discrimination plot with FAM fluorescence signal on the y-axis and VIC fluorescence signal on the x-axis. Software tools enable converting the raw data into genotype calls (e.g., homozygotes and heterozygotes.)
TaqMan® Genotyper Software
- Mouse SNPbrowser™ Software: SNP selection for genetic mapping and genetic monitoring in laboratory mice strains
For research use only. Not intended for any animal or human therapeutic or diagnostic use.