MicroRNA Analysis Using TaqMan® Assays
TaqMan® MicroRNA Assays are innovative tools for miRNA research—from isolation through discovery, profiling, quantitation, validation, and functional analysis.
TaqMan® MicroRNA Assays employ a novel target-specific stem-loop reverse transcription primer to address the challenge of the short length of mature miRNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay–based real-time PCR. Also, the stem-loop structure in the tail of the primer confers a key advantage to these assays: specific detection of the mature, biologically active miRNA.
Use our Search Tool below to find your assays.
miRBase v.20 update: Our Content is now aligned with release 20 of the miRBase database and provides comprehensive coverage for the 206 species listed.
Download: Complete list of TaqMan® MicroRNA Assays
Download: Protocol for multiplexing RT and preamplification reactions with TaqMan® MicroRNA Assays
For miRNAs not included in miRBase and for any other Small RNAs, we offer custom designed Custom TaqMan® Small RNA Assay.
For noncoding human, mouse, or rat transcripts longer than 200 nucleotides, we offer TaqMan® Non-coding RNA Assays.
Use our Search Tool below to find the single tube assays you need.
Assay Search Tool:
TaqMan® Pri-miRNA Assays
TaqMan® Pri-miRNA Assays for detecting and measuring primary miRNAs from human, mouse, or rat species.
TaqMan® Non-coding RNA Assays
TaqMan® Non-coding RNA Assays for reliable detection and quantitation of noncoding human, mouse, or rat transcripts longer than 200 nucleotides.
Custom TaqMan® Small RNA Assays
Custom assays to quantitate small RNA are ideal to quantitate your small RNA when a predesigned assay is unavailable—all you need is the sequence of interest.
Order your TaqMan® MicroRNA Assays preplated into Fast or standard 96-well plates with the TaqMan® Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.
384-Well Microfluidic Cards
384-well cards are ideal for profiling studies across a small number of samples (up to 8 samples per card) or with limited sample material (each reaction requires only 2 µL of sample) and are compatible with Applied Biosystems® ViiA™ 7 and 7900HT Real-Time PCR Systems.
Order your TaqMan® MicroRNA Assays preplated into Fast or standard 384-well plates with the TaqMan® Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.
These plates accommodate 3,072 reactions at once, delivering the high-throughput capabilities required for large profiling studies. Designed for use with the Applied Biosystems® OpenArray® Real-Time PCR System and the new QuantStudio™ 12K Flex Real-Time PCR System.
- Specifications and Documentation for TaqMan® MicroRNA Assays
- Which TaqMan® MicroRNA or Non-Coding RNA Assay is right for you?
- MicroRNA and Non-Coding RNA Applications for Real-Time PCR
Regulatory Status: For Research Use Only. Not for human or animal therapeutic or diagnostic use.