TaqMan™ MicroRNA Assays are innovative tools for miRNA research—from isolation through discovery, profiling, quantitation, validation and functional analysis.

There are now even more choices for detection of mature, biologically active miRNA, designed to support a growing range of miRNA applications. The new TaqMan™ Advanced miRNA Assays use ligation-based universal reverse transcription for a streamlined and highly sensitive workflow. The standard TaqMan MicroRNA Assays, which employ target-specific stem-loop reverse transcription primers for 3’ extended templates, continue to cover a range of species using standard TaqMan Assay–based real-time PCR.

See the new TaqMan Advanced miRNA Assays

New TaqMan Advanced miRNA Assays use universal reverse transcription and provide highly sensitive quantification.

Standard TaqMan MicroRNA Assays use a target-specific stem-loop reverse transcription primer.

TaqMan™ Pri-miRNA Assays detect and measure primary miRNAs from human, mouse, or rat species.

Assay search tool - find & buy your single tube TaqMan Assays:

miRBase v.21 Update

The content for our standard TaqMan MicroRNA Assays and the new TaqMan Advanced miRNA Assays is aligned with release 21 of the miRBase database. For a complete list of standard TaqMan MicroRNA Assays, TaqMan Advanced miRNA Assays, and other technical resources, please visit our TaqMan MicroRNA Assays resource page.

Additional miRNA assays by type

TaqMan™ Non-coding RNA Assays for reliable detection and quantitation of noncoding human, mouse, or rat transcripts longer than 200 nucleotides.

Custom assays to quantitate small RNA are ideal to quantitate your small RNA when a predesigned assay is unavailable—all you need is the sequence of interest.

miRNA assays by format

Order your TaqMan MicroRNA Assays preplated into Fast or standard 96-well plates with the TaqMan™ Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.

384-well cards are ideal for profiling studies across a small number of samples (up to 8 samples per card) or with limited sample material (each reaction requires only 2 µL of sample) and are compatible with Applied Biosystems™ ViiA™ 7 and 7900HT real-time PCR systems.

Order your TaqMan® MicroRNA Assays preplated into Fast or standard 384-well plates with the TaqManCustom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.

These plates accommodate 3,072 reactions at once, delivering the high-throughput capabilities required for large profiling studies. Designed for use with the Applied Biosystems OpenArray™ Real-Time PCR System and the new QuantStudio™ 12K Flex Real-Time PCR System.

 

Video: Find the Right TaqMan MicroRNA Assay

It's never been easier to search over 23,000 TaqMan MicroRNA Assays with the new Assay Search tool from Thermo Fisher Scientific. Watch this short video to see how easy it is to find the right TaqMan MicroRNA Assay.

TaqMan® Multiplex qPCR solution
Real-Time PCR Master Mix