TaqMan® MicroRNA Assays

TaqMan® MicroRNA Assays are innovative tools for miRNA research—from isolation through discovery, profiling, quantitation, validation, and functional analysis.

TaqMan® MicroRNA Assays employ a novel target-specific stem-loop reverse transcription primer to address the challenge of the short length of mature miRNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay–based real-time PCR. Also, the stem-loop structure in the tail of the primer confers a key advantage to these assays: specific detection of the mature, biologically active miRNA.

Assay search tool - find & buy your single tube TaqMan® Assays:

miRBase v.20 Update

Our Content is now aligned with release 20 of the miRBase database and provides comprehensive coverage for the 206 species listed. For a complete list of TaqMan® MicroRNA Assays and other technical resources, please visit our TaqMan® MicroRNA Assays resource page.

TaqMan® Pri-miRNA Assays for detecting and measuring primary miRNAs from human, mouse, or rat species.

TaqMan® Non-coding RNA Assays for reliable detection and quantitation of noncoding human, mouse, or rat transcripts longer than 200 nucleotides.

Custom assays to quantitate small RNA are ideal to quantitate your small RNA when a predesigned assay is unavailable—all you need is the sequence of interest.

All formats and custom designed assays

Order your TaqMan® MicroRNA Assays preplated into Fast or standard 96-well plates with the TaqMan® Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.

384-well cards are ideal for profiling studies across a small number of samples (up to 8 samples per card) or with limited sample material (each reaction requires only 2 µL of sample) and are compatible with Applied Biosystems® ViiA™ 7 and 7900HT Real-Time PCR Systems.

Order your TaqMan® MicroRNA Assays preplated into Fast or standard 384-well plates with the TaqMan® Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.

These plates accommodate 3,072 reactions at once, delivering the high-throughput capabilities required for large profiling studies. Designed for use with the Applied Biosystems® OpenArray® Real-Time PCR System and the new QuantStudio™ 12K Flex Real-Time PCR System.

Find the Right TaqMan® MicroRNA Assay

It's never been easier to search the more than 4.5 million TaqMan® MicroRNA Assays with the new Assay Search tool from Life Technologies. Watch this short video to see how easy it is to find the right TaqMan® MicroRNA Assay.

TaqMan® Multiplex qPCR solution
Real-Time PCR Master Mix